Molecular Cloning, Nucleotide Sequencing, and Characterization of a 27-kDa Antigenic Protein from Paracoccidioides brasiliensis

dc.creatorMcEwen Ochoa, Juan Guillermo
dc.creatorOrtíz Reyes, Blanca Lucia
dc.creatorGarcía Cepero, Ana María
dc.creatorFlórez, Álvaro Mauricio
dc.creatorBotero, Silvia
dc.creatorRestrepo Moreno, Angela
dc.date2023-03-02T16:56:27Z
dc.date2023-03-02T16:56:27Z
dc.date1996
dc.date.accessioned2024-04-23T14:02:31Z
dc.date.available2024-04-23T14:02:31Z
dc.identifier1087-1845
dc.identifierhttps://hdl.handle.net/10495/33655
dc.identifier10.1006/fgbi.1996.0027
dc.identifier1096-0937
dc.identifierhttps://www.sciencedirect.com/science/article/pii/S1087184596900274#!
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9228916
dc.descriptionABSTRACT: A gene encoding a 27-kDa antigenic protein fromParacoccidioides brasiliensiswas cloned, sequenced, and characterized. A cDNA library of the mycelial phase was produced and packed in Uni-Zap-XR vector, λ Zap II synthesis kit (Stratagene, La Jolla, CA). The screening of the library was carried out using a pool of sera from paracoccidioidomycosis patients that had proven reactive in serological testing. Among 44,000 immunoscreened clones from the library, 2 were positive (clones 2 and 3). The former was not characterized further. The latter has a 1-kb DNA insert with an open reading frame encoding a protein of 259 amino acids with a predicted molecular mass of 28.6 kDa (27 kDa by SDS–PAGE). This protein corresponds to a 25-kDa protein in antigenic preparations ofP. brasiliensisas determined by Western blot analysis. Comparison of the transcribed sequence with different gene banks failed to reveal a high degree of homology with other proteins. The cloned DNA fragment was easily expressed inEscherichia coliwithout the need of induction by isopropyl-β-D-thiogalactopyranoside. These findings suggest that the gene encodes aP. brasiliensis-specific protein.
dc.descriptionCOL0000962
dc.format7
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.publisherBiología Celular y Molecular CIB U. de A. U. del Rosario
dc.publisherOrlando, Estados Unidos
dc.relationFungal Genet. Biol.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCloning, Molecular
dc.subjectClonación Molecular
dc.subjectHigh-Throughput Nucleotide Sequencing
dc.subjectSecuenciación de Nucleótidos de Alto Rendimiento
dc.subjectParacoccidioides
dc.titleMolecular Cloning, Nucleotide Sequencing, and Characterization of a 27-kDa Antigenic Protein from Paracoccidioides brasiliensis
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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