Development and Validation of A Stability-Indicating Mekc Method for Determination of Flucloxacillin Sodium in Capsules

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMasquio Fiorentino, Flavia Angelica
dc.creatorSangoi, Maximiliano da Silva
dc.creatorSteppe, Martin
dc.creatorSalgado, Hérida Regina Nunes
dc.date2014-12-03T13:09:06Z
dc.date2016-10-25T20:10:04Z
dc.date2014-12-03T13:09:06Z
dc.date2016-10-25T20:10:04Z
dc.date2014-01-01
dc.date.accessioned2017-04-06T06:17:51Z
dc.date.available2017-04-06T06:17:51Z
dc.identifierCurrent Analytical Chemistry. Sharjah: Bentham Science Publ Ltd, v. 10, n. 1, p. 149-157, 2014.
dc.identifier1573-4110
dc.identifierhttp://hdl.handle.net/11449/111945
dc.identifierhttp://acervodigital.unesp.br/handle/11449/111945
dc.identifierWOS:000326245500012
dc.identifierhttp://dx.doi.org/10.2174/15734110113099990016
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/922716
dc.descriptionFlucloxacillin (FLU) is a semi-synthetic penicillin active against Gram-positive bacteria such as streptococci and penicilinase-producing staphylococci, including methicillin-susceptible S. aureus. A simple, rapid and reproducible stability-indicating micellar electrokinetic chromatography (MEKC) method was developed and validated for analysis of flucloxacillin sodium in capsules, using nimesulide as internal standard. The MEKC method was performed on a fused-silica capillary (50 mu m id; effective length of 40 cm). The background electrolyte consists of 50 mM borate and 80 mM anionic surfactant SDS quantities at pH 8.5. The separation was achieved at 20 kV applied voltage and 27.5 degrees C. The injection was performed using the hydrodinamic mode at 50 mbar for 4s, with detection at 210 nm. The method was linear in the range of 20-100 mu g mL(-1) (r = 0.9996) with adequate results for the precision (<= 1.60%) and accuracy (99.45%). The specificity and stability-indicating capability were demonstrated through forced degradation studies and with placebo solution, which showed that there is no interference of the excipients. LOD and LOQ were 3.20 and 9.70 mu g mL(-1), respectively. The method proved to be robust by a fractional factorial design evaluation. The method was successfully applied for the drug analysis and the results were compared to liquid chromatographic method, showing non-significant difference (p > 0.005). The proposed method might be applied in routine quality control in the pharmaceutical industries.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherBentham Science Publ Ltd
dc.relationCurrent Analytical Chemistry
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCapsules
dc.subjectflucloxacillin sodium
dc.subjectMEKC method
dc.subjectpenicillin
dc.subjectquality control
dc.subjectvalidation
dc.titleDevelopment and Validation of A Stability-Indicating Mekc Method for Determination of Flucloxacillin Sodium in Capsules
dc.typeOtro


Este ítem pertenece a la siguiente institución