A high-fat diet increases interleukin-3 and granulocyte colony-stimulating factor production by bone marrow cells and triggers bone marrow hyperplasia and neutrophilia in wistar rats

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCarmo, Luciana Simao do
dc.creatorRogero, Marcelo Macedo
dc.creatorParedes-Gamero, Edgar Julian
dc.creatorNogueira-Pedro, Amanda
dc.creatorXavier, Jose Guilherme
dc.creatorCortez, Mayara
dc.creatorBorges, Maria Carolina
dc.creatorFujii, Tatiane Mieko
dc.creatorBorelli, Primavera
dc.creatorFock, Ricardo Ambrosio
dc.date2014-12-03T13:08:51Z
dc.date2016-10-25T20:09:23Z
dc.date2014-12-03T13:08:51Z
dc.date2016-10-25T20:09:23Z
dc.date2013-04-01
dc.date.accessioned2017-04-06T06:15:03Z
dc.date.available2017-04-06T06:15:03Z
dc.identifierExperimental Biology And Medicine. London: Royal Soc Medicine Press Ltd, v. 238, n. 4, p. 375-384, 2013.
dc.identifier1535-3702
dc.identifierhttp://hdl.handle.net/11449/111641
dc.identifierhttp://acervodigital.unesp.br/handle/11449/111641
dc.identifier10.1177/1535370213477976
dc.identifierWOS:000321757900006
dc.identifierhttp://dx.doi.org/10.1177/1535370213477976
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/922415
dc.descriptionIt is well established that the excessive consumption of a high-fat diet (HFD) results in overweight, obesity and an increase in leptin concentrations, which triggers a chronic inflammatory condition that is associated with a high white blood cell count. Two-month-old male Wistar rats were fed a control (CON) diet or an HFD for 12 weeks. After this period, hemogram, myelogram and biochemical parameters were evaluated along with the cell cycle and the percentage of CD34(+) cells in the bone marrow as well as cell proliferation and differentiation assays and the production of stem cell factor, interleukin 3 (IL-3), granulocyte colony-stimulating factor (G-CSF) and granulocyte macrophage colony-stimulating factor (GM-CSF). The HFD animals exhibited leukocytosis and neutrophilia with increased C-reactive protein, leptin, cholesterol and triglyceride concentrations. In the HFD group, the bone marrow revealed myeloid hyperplasia, especially of the granulocytic compartment with a higher percentage of CD34(+) cells and a higher percentage of cells in the G2/S/M cell cycle phases. In addition, the HFD bone marrow cells had a higher capacity to proliferate and differentiate into granulocytic cells in an in vitro system and a higher capacity to produce IL-3 and G-CSF. These data led us to infer that the HFD induces leukocytosis and neutrophilia suggesting alterations in hematopoiesis system modulation.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherRoyal Soc Medicine Press Ltd
dc.relationExperimental Biology and Medicine
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjecthigh-fat diet
dc.subjectleukocytosis
dc.subjectneutrophilia
dc.subjectIL-3
dc.subjectG-CSF
dc.subjectCD34 cells
dc.titleA high-fat diet increases interleukin-3 and granulocyte colony-stimulating factor production by bone marrow cells and triggers bone marrow hyperplasia and neutrophilia in wistar rats
dc.typeOtro


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