Penicillinase-based amperometric biosensor for penicillin G

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGoncalves, Luis Moreira
dc.creatorCallera, Welder F. A.
dc.creatorSotomayor, Maria Del Pilar Taboada
dc.creatorBueno, Paulo Roberto
dc.date2014-12-03T13:08:38Z
dc.date2016-10-25T20:08:28Z
dc.date2014-12-03T13:08:38Z
dc.date2016-10-25T20:08:28Z
dc.date2014-01-01
dc.date.accessioned2017-04-06T06:12:55Z
dc.date.available2017-04-06T06:12:55Z
dc.identifierElectrochemistry Communications. New York: Elsevier Science Inc, v. 38, p. 131-133, 2014.
dc.identifier1388-2481
dc.identifierhttp://hdl.handle.net/11449/111420
dc.identifierhttp://acervodigital.unesp.br/handle/11449/111420
dc.identifier10.1016/j.elecom.2013.11.022
dc.identifierWOS:000331504300035
dc.identifierhttp://dx.doi.org/10.1016/j.elecom.2013.11.022
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/922199
dc.descriptionA biosensor for penicillin G was created by immobilizing penicillinase to a gold electrode by means of a cysteine self-assembled monolayer. The biosensor amperometrically monitored the catalytic hydrolysis of penicillin in a very sensible manner. Furthermore, it was successfully used to measure the Michaelis-Menten enzymatic constant and a low limit of detection of 4.5 nM was obtained. (C) 2013 Elsevier B.V. All rights reserved.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherElsevier B.V.
dc.relationElectrochemistry Communications
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCysteine
dc.subjectMetallo-beta-lactamase
dc.subjectSelf-assembled monolayer (SAM)
dc.subjectbeta-Lactam antibiotic
dc.subjectLineweaver-Burk plot
dc.titlePenicillinase-based amperometric biosensor for penicillin G
dc.typeOtro


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