Liquid Nitrogen as promotor of seeds germination and seedling growth in tropical legumes

El nitrógeno líquido como promotor de la germinación de las semillas y del crecimiento de las plántulas en las leguminosas tropicales

dc.creatorAcosta Fernández, Yanier
dc.creatorFontes Marrero, Dayami
dc.creatorMartinez-Montero, Marcos Edel
dc.date2023-05-24T22:25:31Z
dc.date2023-05-24T22:25:31Z
dc.date2021
dc.date.accessioned2023-10-03T20:00:11Z
dc.date.available2023-10-03T20:00:11Z
dc.identifierY. Acosta Fernández, D. Fontes Marrero & M. E. Martínez-Montero, “Liquid Nitrogen as promotor of seeds germination and seedling growth in tropical legumes”, INGE CUC, vol. 17, no. 2, pp. 1–10. DOI: http://doi.org/10.17981/ingecuc.17.2.2021.01
dc.identifier0122-6517
dc.identifierhttps://hdl.handle.net/11323/10177
dc.identifier10.17981/ingecuc.17.2.2021.01
dc.identifier2382-4700
dc.identifierCorporación Universidad de la Costa
dc.identifierREDICUC – Repositorio CUC
dc.identifierhttps://repositorio.cuc.edu.co/
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9173754
dc.descriptionIntroduction— The hard seed is the main cause of dormancy in most of the Leguminoseae species. Seed scarification methods, where physical damage is sought to break hard seed coat without diminishing quality, have been modified over time to make them more effective. The most commonly used seed scarification methods include heat, mechanical scarification, and freeze-thaw. Some methods for freeze-thaw scarification include ultra-low temperature immersion in Liquid Nitrogen (LN, –196°C). Objective— Determine the effectiveness use of Liquid Nitrogen (LN) as a scarification method to overcome dormancy in seeds of species of the Leguminoseae family. Methodology— The physiological quality of all freshly harvested seeds was determined and scarified by direct immersion in LN for 30 minutes. Total germination was determined under laboratory conditions, as well as the time required for the seeds to reach 50% germination (T50) and the total number of seeds that remained hard at the end of the experiment. The percentage of emerged seedlings and their vegetative growth was evaluated for 21 days after sowing. Results— The seeds of all species evaluated showed a high physiological quality at the time of harvest. Scarification with LN improved germination, emergence and vegetative growth in the species Desmodium scorpiorus, Teramnus labialis, Neonotonia wigthii and Phueraria phaseoloides. Conclusions— Dormancy was effectively overcome in the seeds of the species D. scorpiorus, T. labialis, N. wigthii and P. phaseoloides. It was possible to increase the percentage and speed of germination and emergence, managing to obtain plants with greater vegetative growth during the first 21 days after sowing.
dc.descriptionIntroducción— La semilla dura es la principal causa de latencia en la mayoría de las especies de Leguminoseae. Los métodos de escarificación de la semilla, en los que se busca un daño físico para romper la cubierta dura de la semilla sin disminuir su calidad, se han modificado a lo largo del tiempo para hacerlos más efectivos. Los métodos de escarificación de semillas más utilizados son el calor, la escarificación mecánica y la congelación-descongelación. Algunos métodos de escarificación por congelación-descongelación incluyen la inmersión a muy baja temperatura en Nitrógeno Líquido (LN, –196°C). Objetivo— Determinar la efectividad del uso de Nitrógeno Líquido (LN) como método de escarificación para superar la latencia en semillas de especies de la familia Leguminoseae. Metodología— Se determinó la calidad fisiológica de todas las semillas recién cosechadas y se escarificaron por inmersión directa en LN durante 30 minutos. Se determinó la germinación total en condiciones de laboratorio, así como el tiempo necesario para que las semillas alcanzaran el 50% de germinación (T50) y el número total de semillas que permanecieron duras al final del experimento. Se evaluó el porcentaje de plántulas emergidas y su crecimiento vegetativo durante 21 días después de la siembra. Resultados— Las semillas de todas las especies evaluadas mostraron una alta calidad fisiológica en el momento de la cosecha. La escarificación con LN mejoró la germinación, emergencia y crecimiento vegetativo en las especies Desmodium scorpiorus, Teramnus labialis, Neonotonia wigthii y Phueraria phaseoloides. Conclusiones— La dormancia fue superada efectivamente en las semillas de las especies D. scorpiorus, T. labialis, N. wigthii y P. phaseoloides. Se logró aumentar el porcentaje y la velocidad de germinación y emergencia, logrando obtener plantas con mayor crecimiento vegetativo durante los primeros 21 días después de la siembra.
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dc.formatapplication/pdf
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dc.languageeng
dc.publisherCorporación Universidad de la Costa
dc.publisherColombia
dc.relationINGE CUC
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dc.rightsDerechos de autor 2021 INGE CUC
dc.rightsAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.sourcehttps://revistascientificas.cuc.edu.co/ingecuc/article/view/3312
dc.subjectLegumes
dc.subjectSeed dormancy
dc.subjectGermination
dc.subjectLiquid nitrogen
dc.subjectLeguminosas
dc.subjectLatencia de semillas
dc.subjectGerminación
dc.subjectNitrógeno líquido
dc.titleLiquid Nitrogen as promotor of seeds germination and seedling growth in tropical legumes
dc.titleEl nitrógeno líquido como promotor de la germinación de las semillas y del crecimiento de las plántulas en las leguminosas tropicales
dc.typeArtículo de revista
dc.typehttp://purl.org/coar/resource_type/c_6501
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typeText
dc.typeinfo:eu-repo/semantics/article
dc.typehttp://purl.org/redcol/resource_type/ART
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/version/c_970fb48d4fbd8a85


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