Luminescence imaging and toxicity assessment of graphene quantum dots using in vitro models

dc.creatorDe Falco, Anna
dc.creatorSANTA-HELENA, EDUARDA
dc.creatorT. Toloza, Carlos A.
dc.creatorAlmeida, Joseany
dc.creatorLarrude, Dunieskys G.
dc.creatorPereira Meirelles, Fatima Ventura
dc.creatorGioda, Carolina
dc.creatorAucelio, Ricardo Q.
dc.creatorGioda, Adriana
dc.date2023-02-24T13:57:10Z
dc.date2023
dc.date2023-02-24T13:57:10Z
dc.date2022
dc.date.accessioned2023-10-03T19:48:08Z
dc.date.available2023-10-03T19:48:08Z
dc.identifierAnna De Falco, Eduarda Santa-Helena, Carlos A. T. Toloza, Joseany M. S. Almeida, Dunieskys G. Larrude, Fatima Ventura Pereira Meirelles, Carolina Rosa Gioda, Ricardo Q. Aucelio & Adriana Gioda (2022) Luminescence imaging and toxicity assessment of graphene quantum dots using in vitro models, Fullerenes, Nanotubes and Carbon Nanostructures, 30:6, 657-666, DOI: 10.1080/1536383X.2021.1995367
dc.identifier1536-383X
dc.identifierhttps://hdl.handle.net/11323/9925
dc.identifier10.1080/1536383X.2021.1995367
dc.identifier1536-4046
dc.identifierCorporación Universidad de la Costa
dc.identifierREDICUC - Repositorio CUC
dc.identifierhttps://repositorio.cuc.edu.co/
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9172162
dc.descriptionGraphene quantum dots (GQDs) have been of high interest due to their size and optical characteristics, which improves when functional groups are added to their borders and defects. In this work, the in vitro toxicity of aqueous dispersion of GQDs (w/wo amino-functionalization) was investigated in two different cellular models (S. cerevisiae and H9c2 cell line). Results in yeast suggest that when at up to 25 % volume concentration, the effect of all tested GQDs was only inhibitory, and, in both cellular models, the toxic effect is rigorously dose-dependent. The comparison of IC50 values of all the tested GQDs reveals no significant variations among them, pointing to non-carbonized citric acid as the more toxic precursor. The obtained data suggest that functionalization makes GQDs less toxic, being the one functionalized with thioacetamide slightly more toxic, followed by the ones functionalized with thiourea and glutathione, respectively. Results confirm that their toxicity is characteristics as a whole, and not as the sum of the toxicity of the precursors. In both models, concentrations up to 2 % showed no significant toxicity. Finally, fluorescence microscopy images suggest that GQDs interact with the cellular membrane and enter in the cell, manifesting fluorescent properties.
dc.format11 páginas
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherTaylor and Francis Ltd.
dc.publisherUnited States
dc.relationFullerenes Nanotubes and Carbon Nanostructures
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dc.rightsAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)
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dc.sourcehttps://www.tandfonline.com/doi/full/10.1080/1536383X.2021.1995367
dc.subjectGraphene quantum dots
dc.subjectCardiomyoblast
dc.subjectCytotoxicity
dc.subjectFluorescence imaging
dc.subjectYeast
dc.titleLuminescence imaging and toxicity assessment of graphene quantum dots using in vitro models
dc.typeArtículo de revista
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typeText
dc.typeinfo:eu-repo/semantics/article
dc.typehttp://purl.org/redcol/resource_type/ART
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