A bump-hole strategy for increased stringency of cell-specific metabolic labeling of rna

dc.creatorNguyen, Kim
dc.creatorKubota, Miles
dc.creatorDel Arco, Jon
dc.creatorFeng, Chao
dc.creatorSingha, Monika
dc.creatorBeasley, Samantha
dc.creatorSakr, Jasmine
dc.creatorP. Gandhi, Sunil
dc.creatorBlurton-Jones, Mathew
dc.creatorFernández Lucas, Jesus
dc.creatorC. Spitale, Robert
dc.date2021-02-19T16:50:53Z
dc.date2021-02-19T16:50:53Z
dc.date2020-11-21
dc.date2021-11-21
dc.date.accessioned2023-10-03T19:04:20Z
dc.date.available2023-10-03T19:04:20Z
dc.identifierhttps://hdl.handle.net/11323/7879
dc.identifierhttps://doi.org/10.1021/acschembio.0c00755
dc.identifierCorporación Universidad de la Costa
dc.identifierREDICUC - Repositorio CUC
dc.identifierhttps://repositorio.cuc.edu.co/
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9167389
dc.descriptionProfiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, we design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for cell-specific metabolic labeling of RNA with exceptional specificity and stringency.
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherCorporación Universidad de la Costa
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dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.rightshttp://purl.org/coar/access_right/c_f1cf
dc.sourceACS Chemical Biology
dc.sourcehttps://pubs.acs.org/doi/10.1021/acschembio.0c00755#
dc.subjectPeptides and proteins
dc.subjectGenetics
dc.subjectLabeling
dc.subjectUracil
dc.subjectImaging probes
dc.titleA bump-hole strategy for increased stringency of cell-specific metabolic labeling of rna
dc.typeArtículo de revista
dc.typehttp://purl.org/coar/resource_type/c_6501
dc.typeText
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/redcol/resource_type/ART
dc.typeinfo:eu-repo/semantics/acceptedVersion
dc.typehttp://purl.org/coar/version/c_ab4af688f83e57aa


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