Detecção de inibidores de proteases em sementes de Punica granatum

Detection of protease inhibitors in seeds of Punica granatum

dc.creatorColares, Lara Franca
dc.creatorSantos Neto, Antero Ricardo
dc.creatorCordeiro, Isac Henrique
dc.creatorCastro, Sandra Bertelli Ribeiro de
dc.creatorVerly, Rodrigo Moreira
dc.creatorAlves, Caio Cesar de Souza
dc.creatorCarli, Alessandra de Paula
dc.date2018-09-06T12:00:07Z
dc.date2018-08-15
dc.date2018-09-06T12:00:07Z
dc.date2017-04
dc.date.accessioned2023-09-29T15:16:49Z
dc.date.available2023-09-29T15:16:49Z
dc.identifierCOLARES, Lara Franca et al .Detecção de inibidores de proteases em sementes de Punica granatum. Quím. Nova, São Paulo, v. 40, n. 3, p. 270-274, abr. 2017. Disponível em <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000300270&lng=pt&nrm=iso>. acessos em 03 set. 2018. http://dx.doi.org/10.21577/0100-4042.20160189.
dc.identifierhttp://dx.doi.org/10.21577/0100-4042.20160189
dc.identifierhttps://repositorio.ufjf.br/jspui/handle/ufjf/7339
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9127852
dc.descriptionThe use of plants as a way to prevent and treat disease comes from ancient times. With the increasing return on consumption of plants for medicinal purposes, the plant-based medicines have gained greater appreciation. Protease inhibitors are compounds that can decrease activity of an enzyme. They are found in plants, especially in Fabaceae, Poaceae, and Solanaceae families, and show satisfactory use to treat diseases due to their regulatory activities and selective proteolytic degradation of target substrates. This study aimed to obtain protease inhibitors in the seeds of Punica granatum, due its use in traditional medicine. The alcohol-acid solution was used to extract and isolate the protease inhibitors in P. granatum seeds and Glycine max grains. The extract of P. granatum seeds showed similar concentration of protease inhibitors when compared to G. max. A 14 kDa band was detected by electrophoresis on polyacrylamide gel, which was characterized by high-performance liquid chromatography as protease inhibitors. The extract of P. granatum seeds showed a potent trypsin inhibitor activity (EC50 18.2 ng mL-1). This study showed for the first time the extraction, purification, and identification of protease inhibitors in Punica granatum seeds.
dc.description-
dc.formatapplication/pdf
dc.languageeng
dc.publisher-
dc.publisherBrasil
dc.publisher-
dc.relationQuímica Nova
dc.rightsAcesso Aberto
dc.subjectProtease inhibitors
dc.subjectPunica granatum seeds
dc.subjectTraditional medicine
dc.subject-
dc.titleDetecção de inibidores de proteases em sementes de Punica granatum
dc.titleDetection of protease inhibitors in seeds of Punica granatum
dc.typeArtigo de Periódico


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