SCREENING OF FECAL SAMPLES FROM ASYMPTOMATIC CHILDREN, FOR NOROVIRUS DETECTION, USING A THIRD GENERATION ENZYME IMMUNOASSAY COMMERCIAL KIT

SCREENING OF FECAL SAMPLES FROM ASYMPTOMATIC CHILDREN, FOR NOROVIRUS DETECTION, USING A THIRD GENERATION ENZYME IMMUNOASSAY COMMERCIAL KIT

dc.creatorPereira Santos, Hugo César
dc.creatorTurones, Larissa Córdova
dc.creatorFiaccadori, Fabíola Souza
dc.creatorPaula Cardoso, Divina das Dôres de
dc.creatorDias e Souza, Menira Borges de Lima
dc.date2014-07-16
dc.date.accessioned2023-09-29T11:48:44Z
dc.date.available2023-09-29T11:48:44Z
dc.identifierhttps://revistas.ufg.br/iptsp/article/view/31111
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9095583
dc.descriptionNorovirus is the leading cause of non-bacterial acute gastroenteritis outbreaks worldwide. Recently, third generation Enzyme Immunoassay (EIA) commercial kits have been developed, and controversial results have been obtained by different studies regarding the sensitivity and specificity of these assays. Therefore, the aim of this study was to test 60 fecal samples, previously tested as positive by RT-PCR for caliciviruses (40 norovirus-positive and 20 sapovirus-positive samples), for qualitative determination of genogroup I and II noroviruses by a commercial EIA kit (RIDASCREEN® Norovirus (C1401) 3rd Generation, R-Biopharm, Darmstadt, Germany). The samples were obtained from 30 children aged less than five years, mostly asymptomatic, who attend a day-care center in Goiânia, Goiás, Brazil. The results conferred a positivity rate for NoV of 35% and a specificity rate of 100% for the EIA, when compared to the RT-PCR. The test also failed to detect samples that were positive for GI.1 and GI.4 norovirus. The presumably lower viral load of asymptomatic children might be related to the poor sensitivity. Our results reinforce the notion thatscreening of samples by molecular assays, especially of samples that might have a low number of viral particles such as those obtained from asymptomatic patients, should not be replaced by the use of EIA kits.en-US
dc.descriptionNorovirus is the leading cause of non-bacterial acute gastroenteritis outbreaks worldwide. Recently, third generation Enzyme Immunoassay (EIA) commercial kits have been developed, and controversial results have been obtained by different studies regarding the sensitivity and specificity of these assays. Therefore, the aim of this study was to test 60 fecal samples, previously tested as positive by RT-PCR for caliciviruses (40 norovirus-positive and 20 sapovirus-positive samples), for qualitative determination of genogroup I and II noroviruses by a commercial EIA kit (RIDASCREEN® Norovirus (C1401) 3rd Generation, R-Biopharm, Darmstadt, Germany). The samples were obtained from 30 children aged less than five years, mostly asymptomatic, who attend a day-care center in Goiânia, Goiás, Brazil. The results conferred a positivity rate for NoV of 35% and a specificity rate of 100% for the EIA, when compared to the RT-PCR. The test also failed to detect samples that were positive for GI.1 and GI.4 norovirus. The presumably lower viral load of asymptomatic children might be related to the poor sensitivity. Our results reinforce the notion thatscreening of samples by molecular assays, especially of samples that might have a low number of viral particles such as those obtained from asymptomatic patients, should not be replaced by the use of EIA kits.pt-BR
dc.formatapplication/pdf
dc.languagepor
dc.publisherUniversidade Federal de Goiáspt-BR
dc.relationhttps://revistas.ufg.br/iptsp/article/view/31111/16786
dc.sourceRevista de Patologia Tropical / Journal of Tropical Pathology; Vol. 43 No. 2 (2014); 143-149en-US
dc.sourceRevista de Patologia Tropical / Journal of Tropical Pathology; v. 43 n. 2 (2014); 143-149pt-BR
dc.source1980-8178
dc.source0301-0406
dc.subjectAsymptomatic childrenen-US
dc.subjectenzyme immunoassayen-US
dc.subjectNorovirusen-US
dc.subjectsensitivityen-US
dc.subjectspecificity.en-US
dc.subjectAsymptomatic childrenpt-BR
dc.subjectenzyme immunoassaypt-BR
dc.subjectNoroviruspt-BR
dc.subjectsensitivitypt-BR
dc.subjectspecificitypt-BR
dc.titleSCREENING OF FECAL SAMPLES FROM ASYMPTOMATIC CHILDREN, FOR NOROVIRUS DETECTION, USING A THIRD GENERATION ENZYME IMMUNOASSAY COMMERCIAL KITen-US
dc.titleSCREENING OF FECAL SAMPLES FROM ASYMPTOMATIC CHILDREN, FOR NOROVIRUS DETECTION, USING A THIRD GENERATION ENZYME IMMUNOASSAY COMMERCIAL KITpt-BR
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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