Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis

dc.creatorBotelho, Deila Magna dos Santos
dc.creatorResende, Mário Lúcio Vilela de
dc.creatorTeixeira, Alexandre Rezende
dc.creatorSantiago, Wilder Douglas
dc.creatorPozza, Edson Ampélio
dc.creatorMoreira, Silvino Intra
dc.creatorRamos, Juliana Barros
dc.creatorAquino, Sinara Oliveira de
dc.creatorFerreira, Antônio Gilberto
dc.creatorReichel, Tharyn
dc.creatorGuerra-Guimarães, Leonor
dc.date2022-07-22T20:55:06Z
dc.date2022-07-22T20:55:06Z
dc.date2022
dc.date.accessioned2023-09-28T20:08:12Z
dc.date.available2023-09-28T20:08:12Z
dc.identifierBOTELHO, D. M. dos S. et al. Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis. Chemical Papers, [S.l.], v. 76, p. 2567-2572, 2022.
dc.identifierhttps://link.springer.com/article/10.1007/s11696-021-01965-5
dc.identifierhttp://repositorio.ufla.br/jspui/handle/1/50700
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9045326
dc.descriptionCercosporin has excellent properties of photosensitization that have been widely used in organophotocatalyst and photodynamic therapy as well as an antimicrobial agent. Therefore, there is a need to quantify it accurately with accessible methods. A comparative analysis of cercosporin quantification obtained by spectrophotometry (SPEC) and high-performance liquid chromatography (HPLC) was performed for nineteen Cercospora coffeicola isolates from different coffee-producing municipalities in Brazil. Image analysis of cercosporin crystals was performed in isolates with either high or low production of the toxin. Our results show that SPEC and HPLC are equally valid for the cercosporin evaluation of C. coffeicola cultures grown in vitro. The isolates with high cercosporin production had a higher crystal number and size when compared to the one with low cercosporin production.
dc.languageen_US
dc.publisherSpringer
dc.rightsrestrictAccess
dc.sourceChemical Papers
dc.subjectMicroscopy
dc.subjectToxin
dc.subjectHigh-performance liquid chromatography
dc.subjectMethods
dc.titleCercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis
dc.typeArtigo


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