Optimal cytokinin/auxin balance for indirect shoot organogenesis of Eucalyptus cloeziana and production of ex vitro rooted micro-cuttings

dc.creatorOliveira, Leandro Silva de
dc.creatorBrondani, Gilvano Ebling
dc.creatorMolinari, Letícia Vaz
dc.creatorDias, Rafaella Zanetti
dc.creatorTeixeira, Gustavo Leal
dc.creatorGonçalves, Antônio Natal
dc.creatorAlmeida, Marcílio de
dc.date2022-08-09T19:39:59Z
dc.date2022-08-09T19:39:59Z
dc.date2022
dc.date.accessioned2023-09-28T20:08:01Z
dc.date.available2023-09-28T20:08:01Z
dc.identifierOLIVEIRA, L. S. de et al. Optimal cytokinin/auxin balance for indirect shoot organogenesis of Eucalyptus cloeziana and production of ex vitro rooted micro-cuttings. Journal of Forestry Research, [S.l.], 2022.
dc.identifierhttps://link.springer.com/article/10.1007/s11676-022-01454-9
dc.identifierhttp://repositorio.ufla.br/jspui/handle/1/50909
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9045248
dc.descriptionShoot organogenesis is critical for the shortening of long breeding cycles and circumvent the barrier of cloning mature Eucalyptus cloeziana trees. It enables large-scale production of plants from transformed tissues. This study evaluates the effect of α-naphthaleneacetic acid (NAA), thidiazuron (TDZ) and benzylaminopurine (BAP) on the organogenesis of E. cloeziana from hypocotyls and cotyledonary leaves. In the induction stage, hypocotyls and cotyledonary leaves were established in a Murashige and Skoog (MS) culture medium supplemented with NAA or TDZ. Callus tissues were cultivated in a MS culture medium containing only BAP or different concentrations of BAP/NAA in the differentiation stage. Adventitious buds were multiplied in vitro and elongated in a WPM culture medium supplemented with 0.89 µM BAP and 0.05 µM NAA. Cotyledonary leaves exhibited the best in vitro regeneration. The induction of adventitious buds occurred only in calluses induced from TDZ. In the differentiation stage, 4.4 µM BAP treatment promoted an increase of adventitious bud regeneration. Micro-cuttings from regenerated shoots were acclimatized and rooted ex vitro in mini-incubators. The results confirm the establishment of an efficient protocol for the in vitro regeneration of E. cloeziana by indirect organogenesis, providing new insights regarding cloning of this species.
dc.languageen_US
dc.publisherSpringer
dc.rightsrestrictAccess
dc.sourceJournal of Forestry Research
dc.subjectMicropropagation
dc.subjectRecalcitrant species
dc.subjectPlant growth regulator
dc.subjectExplants source
dc.titleOptimal cytokinin/auxin balance for indirect shoot organogenesis of Eucalyptus cloeziana and production of ex vitro rooted micro-cuttings
dc.typeArtigo


Este ítem pertenece a la siguiente institución