Validation and estimation of uncertainty of an LC-MS/MS method for the simultaneous determination of 34 mycotoxins in cocoa beans

dc.creatorAbreu, Daiane Cássia Pereira
dc.creatorVargas, Eugenia Azevedo
dc.creatorOliveira, Fabiano Aurélio da Silva
dc.creatorMadureira, Fernando Diniz
dc.creatorGomes, Mateus Batista
dc.creatorBazzana, Matheus Julien Ferreira
dc.creatorSaczk, Adelir Aparecida
dc.date2023-04-19T16:40:37Z
dc.date2023-04-19T16:40:37Z
dc.date2023
dc.date.accessioned2023-09-28T20:07:34Z
dc.date.available2023-09-28T20:07:34Z
dc.identifierABREU, D. C. P. et al. Validation and estimation of uncertainty of an LC-MS/MS method for the simultaneous determination of 34 mycotoxins in cocoa beans. Food Chemistry, [S.l.], v. 399, 2023.
dc.identifierhttps://www.sciencedirect.com/science/article/pii/S0308814622018647
dc.identifierhttp://repositorio.ufla.br/jspui/handle/1/56683
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9045061
dc.descriptionThe aim of this manuscript was to validate and apply an analytical methodology for the simultaneous determination of 34 mycotoxins in cocoa. The extraction method used in the tests was a liquid–liquid partition by NaCl addition with a freezing step followed by quantification using LC-MS/MS. The results were discussed based on national and international directives for food contaminants. The recoveries and precision were adequate, except for the mycotoxins ionized with the ammonium adduct (NH4+), E-cristinine and β-ZOL. This result directly influenced the measurement uncertainty of these mycotoxins, because the precision and the correction factor of the recovery were the factors with the greatest impact on the uncertainty of the method. The evaluation of the matrix effect showed considerable signal suppression for 53 % of the evaluated mycotoxins. Nevertheless, the mycotoxins exhibited relatively low quantification limits, with values between 1 and 75 μg kg−1. The validated methodology was applied to 15 cocoa samples collected in warehouses in Brazil. Positive results were found for all the evaluated samples, in which nine toxins were detected out of the 34 investigated.
dc.languageen_US
dc.publisherElsevier
dc.rightsrestrictAccess
dc.sourceFood Chemistry
dc.subjectMulti-mycotoxin
dc.subjectFood safety
dc.subjectSample preparation
dc.titleValidation and estimation of uncertainty of an LC-MS/MS method for the simultaneous determination of 34 mycotoxins in cocoa beans
dc.typeArtigo


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