| dc.creator | Santos, Gustavo Costa | |
| dc.creator | Von Pinho, Edila Vilela de Resende | |
| dc.creator | Rosa, Sttela Dellyzete Veiga Franco da | |
| dc.date | 2017-12-18T11:13:08Z | |
| dc.date | 2017-12-18T11:13:08Z | |
| dc.date | 2013 | |
| dc.date.accessioned | 2023-09-28T20:07:07Z | |
| dc.date.available | 2023-09-28T20:07:07Z | |
| dc.identifier | SANTOS, G. C.; VON PINHO, E. V. de R.; ROSA, S. D. V. F. da. Gene expression of coffee seed oxidation and germination processes during drying. Genetics and Molecular Research, Ribeirão Preto, v. 12, n. 4, p. 6968-6982, 2013. | |
| dc.identifier | https://www.geneticsmr.com/articles/gene-expression-of-coffee-seed-oxidation-and-germination-processes-during-drying.pdf | |
| dc.identifier | http://repositorio.ufla.br/jspui/handle/1/28281 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/9044886 | |
| dc.description | Coffee (Coffea arabica L.) seeds are sensitive
to desiccation and oxidative stress during drying processes. We
investigated the effect of drying and moisture levels on germinationrelated
gene expressions associated with enzymatic systems that
prevent oxidative stress in coffee seeds. Coffee seeds collected at
physiological maturity were subjected to slow and quick drying to
40, 30, 20, and 12% moisture levels (wet basis), and as the control,
seeds without drying were used. The seeds’ physiological quality was
calculated as percentage of normal seedlings at 15 and 30 days, normal
vigorous seedlings at 30 days, and cotyledonary leaves at 45 days. The
isoenzymes esterase, catalase (CAT), peroxidase (POX), and endo-β-
mannanase expressions were electrophoretically analyzed. CAT and
POX expressions were analyzed using RT-qPCR with specific primers
constructed from the target gene sequences from the Brazilian Coffee
Genome Database. Slow drying showed better physiological quality
for seeds at 40 and 12% moisture levels, while quick drying was
the most effective for seeds with 20% moisture. Sensitivity to water
loss was confirmed by quick drying and activation of enzymes. CAT
and POX transcriptions reduced during drying. RT-qPCR revealed a complex gene-expression pattern during the oxidative process, with
high gene expression in wet seeds. | |
| dc.language | en_US | |
| dc.publisher | Fundação de Pesquisas Científicas de Ribeirão Preto | |
| dc.rights | restrictAccess | |
| dc.source | Genetics and Molecular Research | |
| dc.subject | Desiccation tolerance | |
| dc.subject | Isoenzyme | |
| dc.subject | Endo-β-mannanase | |
| dc.subject | RT-PCR | |
| dc.subject | Seed aging | |
| dc.title | Gene expression of coffee seed oxidation and germination processes during drying | |
| dc.type | Artigo | |
