Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

dc.creatorLeal, C. A. G.
dc.creatorCarvalho-Castro, G. A.
dc.creatorCottorello, A. C.
dc.creatorLeite, R. C.
dc.creatorFigueiredo, H. C. P.
dc.date2019-11-04T12:53:51Z
dc.date2023-06-27T19:57:30Z
dc.date2019-11-04T12:53:51Z
dc.date2023-06-27T19:57:30Z
dc.date2013
dc.date.accessioned2023-09-28T20:05:26Z
dc.date.available2023-09-28T20:05:26Z
dc.identifierLEAL, C. A. G. et al. Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD. Brazilian Journal of Microbiology, São Paulo, v. 44, n. 3, July/Sept. 2013.
dc.identifierhttp://repositorio.ufla.br/jspui/handle/1/57882
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9044195
dc.descriptionThe aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
dc.languageen_US
dc.publisherSociedade Brasileira de Microbiologia
dc.rightsAttribution-NonCommercial 4.0 International
dc.rightsacesso aberto
dc.rightshttp://creativecommons.org/licenses/by-nc/4.0/
dc.sourceBrazilian Journal of Microbiology
dc.subjectClinical samples
dc.subjectSensitivity
dc.subjectWhite spot syndrome virus (WSSV)
dc.subjectReal-time PCR
dc.subjectWhite spot disease (WSD)
dc.titleComparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
dc.typeArtigo


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