Microbiome-guided evaluation of Bacillus subtilis BIOUFLA2 application to reduce mycotoxins in maize kernels

dc.creatorGuimarães, Rafaela Araújo
dc.creatorPherez-Perrony, Paul Esteban
dc.creatorMüller, Henry
dc.creatorBerg, Gabriele
dc.creatorMedeiros, Flávio Henrique Vasconcelos
dc.creatorCernava, Tomislav
dc.date2020-08-21T16:33:45Z
dc.date2020-08-21T16:33:45Z
dc.date2020-11
dc.date.accessioned2023-09-28T20:05:12Z
dc.date.available2023-09-28T20:05:12Z
dc.identifierGUIMARÃES, R. A. et al. Microbiome-guided evaluation of Bacillus subtilis BIOUFLA2 application to reduce mycotoxins in maize kernels. Biological Control, Orlando, v. 150, Nov. 2020. DOI: https://doi.org/10.1016/j.biocontrol.2020.104370.
dc.identifierhttps://www.sciencedirect.com/science/article/abs/pii/S1049964420303480#!
dc.identifierhttp://repositorio.ufla.br/jspui/handle/1/42529
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9044101
dc.descriptionFungal diseases are aggravating globally in crop cultivation despite of growing inputs of chemical fungicides. Ear rot and fumonisin contamination of kernels caused by Fusarium verticillioides lead to qualitative and quantitative reductions in maize production. In tropical agriculture, high yielding maize currently can only be assured through foliar protection using fungicides due to the high pathogen pressure. However, the use of chemical fungicides alone does not guarantee the protection of kernels. Recently, a tandem application of fungicides with the Bacillus subtilis strain BIOUFLA2 was identified as a promising strategy to control F. verticillioides-caused diseases. We have employed an integrative approach to assess changes in the microbiome of maize subjected to a combination of chemical and biological fungicides and contrasted it to conventional treatments. This was complemented with molecular and analytical monitoring of the pathogen and mycotoxin levels. Two sampling times were included (10 days after application of treatments and upon harvesting) and two maize fields to account for regional differences. A comparative analysis indicated a treatment-specific enrichment of bacterial and fungal OTUs (Operational Taxonomic Units) that were not detectable in the maize microbiome of untreated plants. Both, the chemical and the tandem application of a chemical and biological pesticide resulted in the occurrence of unique OTUs. Samples subjected to these treatments harbored up to 22 additional OTUs. The lowest fumonisin concentration was observed in the treatment that encompassed BIOUFLA2 (field 1 = 0.29 ppm and field 2 = 0.77 ppm), while the conventional fungicide application resulted in the highest mycotoxin concentrations (field 1 = 3.84 ppm and field 2 = 10.02 ppm). Our study provided strong evidence that conventional treatments of maize with fungicides can promote mycotoxin levels in grains and that partial replacement with biologicals can increase the treatment efficiency.
dc.languageen_US
dc.publisherElsevier
dc.rightsrestrictAccess
dc.sourceBiological Control
dc.subjectIntegrated disease management
dc.subjectMycotoxins
dc.subjectMicrobial communities
dc.subjectPesticides
dc.subjectMicrobial recruitment
dc.subjectGestão integrada de doenças
dc.subjectMicotoxinas
dc.subjectComunidades microbianas
dc.subjectPesticidas
dc.subjectRecrutamento microbiano
dc.titleMicrobiome-guided evaluation of Bacillus subtilis BIOUFLA2 application to reduce mycotoxins in maize kernels
dc.typeArtigo


Este ítem pertenece a la siguiente institución