In vitro induction of hexaploid plants from triploid hybrids of Pennisetum purpureum and Pennisetum glaucum

dc.creatorCampos, J. M. S.
dc.creatorDavide, L. C.
dc.creatorSalgado, C. C.
dc.creatorSantos, F. C.
dc.creatorCosta, P. N.
dc.creatorSilva, P. S.
dc.creatorAlves, C. C. S.
dc.creatorViccini, L. F.
dc.creatorPereira, A. V.
dc.date2020-02-12T14:36:05Z
dc.date2020-02-12T14:36:05Z
dc.date2009-02
dc.date.accessioned2023-09-28T20:04:41Z
dc.date.available2023-09-28T20:04:41Z
dc.identifierCAMPOS, J. M. S. et al. In vitro induction of hexaploid plants from triploid hybrids of Pennisetum purpureum and Pennisetum glaucum. Plant Breeding, [S. l.], v. 128, n. 1, p. 101-104, Feb. 2009.
dc.identifierhttps://onlinelibrary.wiley.com/doi/full/10.1111/j.1439-0523.2008.01546.x
dc.identifierhttp://repositorio.ufla.br/jspui/handle/1/39004
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9043882
dc.descriptionThis study describes an efficient in vitro method using colchicine‐treated triploid seedlings of Napiergrass and Pearl millet hybrids to produce hexaploid hybrids and their subsequent identification with flow cytometry. It also describes chromosome count and stomatal morphology and their use to ploidy analysis. Four‐hundred and eighty triploid seeds, representing 12 different hybrids were sterilized and transferred to MS media to induce chromosome doubling. Surviving plants were analysed by flow cytometry. From six triploid (control) and hexaploid plants, the stomata sizes and frequency were analysed. Chromosome count was performed only in the plants identified as hexaploid. Seventeen plants were identified as hexaploid by flow cytometry analysis. Further confirmation of the hexaploid condition was performed with stomatal morphology (stomatal frequency reduction and stomatal length increase) and chromosome count (2n = 6x = 42). Chromosome doubling has numerous applications in Pennisetum breeding. It can be used to restore the fertility of interspecific hybrids and to improve seed size.
dc.languageen_US
dc.publisherBlackwell Verlag
dc.rightsrestrictAccess
dc.sourcePlant Breeding
dc.subjectChromosome doubling
dc.subjectColchicine
dc.subjectPennisetum hybrids
dc.subjectFlow cytometry
dc.subjectDuplicação cromossômica
dc.subjectColchicina
dc.subjectCitometria de fluxo
dc.titleIn vitro induction of hexaploid plants from triploid hybrids of Pennisetum purpureum and Pennisetum glaucum
dc.typeArtigo


Este ítem pertenece a la siguiente institución