Evaluation of two automated and three rapid lateral flow immunoassays for the detection of anti-SARS-CoV-2 antibodies

dc.creatorMontesinos, Isabel
dc.creatorGruson, Damien
dc.creatorKabamba, Benoit
dc.creatorDahma, Hafid
dc.creatorVan den Wijngaert, Sigi
dc.creatorReza, Soleimani
dc.creatorCarbone, Vincenzo
dc.creatorVandenberg, Olivier
dc.creatorGulbis, Beatrice
dc.creatorWolff, Fleur
dc.creatorRodriguez-Villalobos, Hector
dc.date2020-08-17T18:17:10Z
dc.date2020-08-17T18:17:10Z
dc.date2020-07
dc.date.accessioned2023-09-28T20:01:23Z
dc.date.available2023-09-28T20:01:23Z
dc.identifierMONTESINOS, I. et al. Evaluation of two automated and three rapid lateral flow immunoassays for the detection of anti-SARS-CoV-2 antibodies. Journal of Clinical Virology, [S.l.], v. 128, July 2020.
dc.identifierhttps://www.sciencedirect.com/science/article/pii/S1386653220301554
dc.identifierhttp://repositorio.ufla.br/jspui/handle/1/42444
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9042581
dc.descriptionIntroduction Several SARS-CoV-2 immunoassays have been developed recently. The purpose of this study was to assess the performance of five immunoassays for the detection of SARS-CoV-2 antibodies. Methods Two quantitative automated immunoassays (Maglumi™2019-n-Cov IgG and IgM and Euroimmun Anti-SARS-CoV-2 IgG and IgA assays) and three lateral flow rapid tests were performed. This retrospective study included 200 residual sera from patients and healthy volunteers. Case serum samples (n = 128) were obtained from COVID-19 patients confirmed by RT-qPCR and CT-scan. Days since onset of symptoms was collected from their medical records. Control non-SARS-CoV-2 samples (n = 72) were obtained from anonymous stored residual serum samples. Results Maglumi™ IgG/IgM tests showed overall less sensitivity than Euroimmun IgG/IgA test (84.4 % versus 64.3 %). Both tests showed similar specificities of IgG at 99 % and 100 %, respectively. The results from the lateral flow assays were easily interpretable with unambiguous coloured reading bands. The overall sensitivity of the three tests was similar (around 70 %) without any significant differences. The sensitivity of the three lateral flow assays and also of the serological quantitative assays increased during the second week after symptom onset and all reached similar values (91 %–94 %) after 14 days. Conclusion This study shows accurate and equivalent performance of the five serological antibody assays (ELISA, CLIA and three lateral flow tests) in detecting SARS-CoV-2 antibodies 14 days after the onset of COVID-19 symptoms. This is compatible with their application in specific clinical contexts and in determining epidemiological strategies for the COVID-19 pandemic.
dc.languageen_US
dc.publisherElsevier
dc.rightsrestrictAccess
dc.sourceJournal of Clinical Virology
dc.subjectSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
dc.subjectLateral flow assays
dc.subjectImmunoassays
dc.subjectEnzyme-Linked Immunosorbent Assay (ELISA)
dc.subjectChemiluminescence Immunoassay (CLIA)
dc.subjectCOVID-19
dc.titleEvaluation of two automated and three rapid lateral flow immunoassays for the detection of anti-SARS-CoV-2 antibodies
dc.typeArtigo


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