Capture of lysozyme on macroporous cryogels by hydrophobic affinity chromatography

dc.creatorOliveira, Ana Cristina Freitas de
dc.creatorNeves, Isabelle Cistina Oliveira
dc.creatorSaraiva, Jamile Alves Meireles
dc.creatorCarvalho, Marcelle Flores Fernandes de
dc.creatorBatista, Gioanni Aleixo
dc.creatorVeríssimo, Lizzy Ayra Alcântara
dc.creatorResende, Jaime Vilela de
dc.date2020-04-15T17:48:23Z
dc.date2020-04-15T17:48:23Z
dc.date2019
dc.date.accessioned2023-09-28T20:01:16Z
dc.date.available2023-09-28T20:01:16Z
dc.identifierOLIVEIRA, A. C. F. de et al. Capture of lysozyme on macroporous cryogels by hydrophobic affinity chromatography. Separation Science and Technology, [S. l.], 2019. DOI: https://doi.org/10.1080/01496395.2019.1617743.
dc.identifierhttps://www.tandfonline.com/doi/abs/10.1080/01496395.2019.1617743?journalCode=lsst20
dc.identifierhttp://repositorio.ufla.br/jspui/handle/1/40087
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9042546
dc.descriptionDifferent protocols for functionalization of cryogels with L-tryptophan were tested for the lysozyme adsorption. The effect of ionic strength and pH on the adsorptive capacity (q) was investigated. The morphological, thermal, and mechanical properties of the cryogels were determined. A maximum adsorption for lysozyme (58.030 mg g–1) was achieved when 5% (v/v) of glutaraldehyde (at pH 6.0) and no methanol were used during the cryogel functionalization. The hydrophobic cryogel presented a porosity of 0.666. A strong single band at 14.3 kDa was visualized in SDS-PAGE, indicating that the hydrophobic affinity cryogels was efficient for the lysozyme capture.
dc.languageen_US
dc.publisherTaylor & Francis
dc.rightsrestrictAccess
dc.sourceSeparation Science and Technology
dc.subjectCryogels - Functionalization
dc.subjectPreparative chromatography
dc.subjectLysozyme adsorption
dc.subjectHydrophobic affinity
dc.subjectCriogéis - Funcionalização
dc.subjectCromatografia preparativa
dc.subjectAdsorção de lisozima
dc.subjectAfinidade hidrofóbica
dc.titleCapture of lysozyme on macroporous cryogels by hydrophobic affinity chromatography
dc.typeArtigo


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