| dc.creator | Abreu, Daiane Cássia Pereira | |
| dc.creator | Oliveira, Fabiano Aurelio da Silva | |
| dc.creator | Vargas, Eugenia | |
| dc.creator | Madureira, Fernando | |
| dc.creator | Saczk, Adelir Aparecida | |
| dc.date | 2019-07-18T19:46:35Z | |
| dc.date | 2019-07-18T19:46:35Z | |
| dc.date | 2018 | |
| dc.date.accessioned | 2023-09-28T20:00:47Z | |
| dc.date.available | 2023-09-28T20:00:47Z | |
| dc.identifier | ABREU, D. C. P. et al. Dilute-and-shoot and quechers modified in house for determination of multimycotoxins in cocoa. In: BRAZILIAN CONFERENCE ON MASS SPECTROMETRY, 7.; BRAZILIAN PROTEOMICS SOCIETY CONFERENCE, 4., 2018, Rio de Janeiro. Conference Book… [S.l.]: [s.n.], 2018. p. 70. | |
| dc.identifier | http://repositorio.ufla.br/jspui/handle/1/35461 | |
| dc.identifier | http://congresso2018.brmass.com/wp-content/uploads/2018/12/7thBrmassBookvs12022018.pdf | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/9042322 | |
| dc.description | Due to the high complexity of the cocoa matrices, a modification to the dilute-and-shoot and
QuEChERS methods was proposed for the simultaneous analysis of 42 mycotoxins. The modification
allowed an efficient extraction with reduced co-extractives of the matrix without clean up step with LCMS/MS analysis. The ideal condition was: extraction of the cocoa paste with a ratio of 6:4 ACN/H2O
(v/v), followed by partitioning of the extractive phase with addition of 3g of NaCl, stirring on a shaker
table for 60min and freezing for 15min at -70ºC, the mixture was centrifuged and the supernatant
collected for analysis. The performance of the proposed method was evaluated for 35 mycotoxins,
considering the recovery range of 70-120% and RSD less than 20%, 5 mycotoxins presented an
unsatisfactory result one of the levels of fortification. LD's ranged in between 1.3-27.6μg kg-1. The
limits of aflatoxins and ochratoxins defined in the legislation for cocoa were met. | |
| dc.description | http://congresso2018.brmass.com/wp-content/uploads/2018/12/7thBrmassBookvs12022018.pdf | |
| dc.language | en_US | |
| dc.rights | restrictAccess | |
| dc.subject | Liquid chromatography-tandem mass spectrometry (LC-MS/MS) | |
| dc.subject | Food safety | |
| dc.subject | Sample preparation | |
| dc.title | Dilute-and-shoot and quechers modified in house for determination of multimycotoxins in cocoa | |
| dc.type | Trabalho apresentado em evento | |
