Characterization and detection of adulterated whey protein supplements using stationary and time-resolved fluorescence spectroscopy

dc.creatorPereira, Cristina Guimarães
dc.creatorAndrade, Jonathan
dc.creatorRanquine, Thamiris
dc.creatorMoura, Israel Novaes de
dc.creatorRocha, Roney Alves da
dc.creatorFurtado, Marco Antônio Moreira
dc.creatorBell, Maria Jose Valenzuela
dc.creatorAnjos, Virgilio
dc.date2019-06-12T10:50:40Z
dc.date2019-06-12T10:50:40Z
dc.date2018-11
dc.date.accessioned2023-09-28T20:00:23Z
dc.date.available2023-09-28T20:00:23Z
dc.identifierPEREIRA, C. G. et al. Characterization and detection of adulterated whey protein supplements using stationary and time-resolved fluorescence spectroscopy. LWT, [S.l.], v. 97, p. 180-186, Nov. 2018.
dc.identifierhttps://www.sciencedirect.com/science/article/pii/S0023643818305619
dc.identifierhttp://repositorio.ufla.br/jspui/handle/1/34719
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9042164
dc.descriptionThe objective of this work was to evaluate the efficacy of time-resolved and stationary fluorescence spectroscopic techniques in the detection of adulterations in Whey Protein Concentrate (WPC) powder, used in nutritional supplements. Adulterations were performed by the individual addition of caffeine, creatine and lactose in WPC in different levels (10%, 20% and 30% w/w). Samples were submitted to 275 nm excitation wavelength. Time-resolved fluorescence revealed that the adulterations changed the lifetimes of the tri-exponential decay curves obtained at 335 nm. The 30% adulterations demonstrated higher values of the mean emission lifetime, with caffeine treatment statistically significant. The fluorescence spectra showed an emission peak at approximately 335 nm with a broadband between 325 and 430 nm in the caffeine treatment. Euclidean distance analysis indicated the creatine and lactose treatments (10, 20 and 30% w/w) as similar to control WPC, while caffeine 20% and 30% treatments not. Hierarchical Cluster Analysis could differentiate all caffeine treatments from the other samples. Principal Component Analysis confirmed the differentiation of all adulterants from the control WPC. In general, the application of time-resolved fluorescence and stationary fluorescence spectroscopy techniques allowed the characterization and observation of differences among the samples, when allied to statistical tools.
dc.languageen_US
dc.publisherElsevier
dc.rightsrestrictAccess
dc.sourceLWT
dc.subjectWhey protein concentrate
dc.subjectAdulteration
dc.subjectSpectroscopy
dc.subjectFluorescence
dc.subjectTime-resolved fluorescence
dc.titleCharacterization and detection of adulterated whey protein supplements using stationary and time-resolved fluorescence spectroscopy
dc.typeArtigo


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