Spent Pot Liner (SPL) induced DNA damage and nuclear alterations in root tip cells of Allium cepa as a consequence of programmed cell death

dc.creatorAndrade-Vieira, L. F.
dc.creatorGedraite, L. S.
dc.creatorCampos, J. M. S.
dc.creatorDavide, L. C.
dc.date2020-02-05T19:08:02Z
dc.date2020-02-05T19:08:02Z
dc.date2011-05
dc.date.accessioned2023-09-28T19:59:44Z
dc.date.available2023-09-28T19:59:44Z
dc.identifierANDRADE-VIEIRA, L. F.; GEDRAITE, L. S.; CAMPOS, J. M. S.; DAVIDE, L. C. Spent Pot Liner (SPL) induced DNA damage and nuclear alterations in root tip cells of Allium cepa as a consequence of programmed cell death. Ecotoxicology and Environmental Safety, New York, v. 74, n. 4, p. p. 882-884, May 2011.
dc.identifierhttps://www.sciencedirect.com/science/article/pii/S0147651310003933?via%3Dihub#!
dc.identifierhttp://repositorio.ufla.br/jspui/handle/1/38824
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9041904
dc.descriptionThere are various toxic effects of environmental pollutants, including apoptosis and carcinogenesis. Spent Pot Liner (SPL) is solid waste from the aluminum industry. It has a highly variable composition, including cyanide, fluoride, organics and metals. Preliminary characterizations of the effect of SPL on Allium cepa show the presence of condensed nuclei. Thus, the aim of this study was to analyze the toxic effect of SPL in A. cepa root meristem in the context of programmed cell death (PCD). A lot of specific features of this process such as DNA fragmentation, condensed chromatin, spherical nuclei and the formation of apoptotic-like bodies were observed in root meristem after SPL treatment. Root meristem treated with SPL 25% solution exhibited an alteration in antioxidant enzyme activities; a reduction in NCR as a consequence of high percentage of condensed nuclei; DNA fragmentation, detected by electrophoresis and TUNEL assay; cytoplasm vacuolization and also a disturbance in root morphology. These features are associated with programmed cell death (PCD) under abiotic stress. Therefore, these data show that SPL induces apoptosis-like PCD in root meristem cells of A. cepa.
dc.languageen_US
dc.publisherElsevier
dc.rightsrestrictAccess
dc.sourceEcotoxicology and Environmental Safety
dc.subjectSpent pot liner
dc.subjectProgrammed cell death
dc.subjectAntioxidant enzymes
dc.subjectDNA fragmentation
dc.subjectEnvironmental mutagenesis
dc.subjectMorte celular programada
dc.subjectEnzimas antioxidantes
dc.subjectFragmentação do DNA
dc.subjectMutagênese ambiental
dc.titleSpent Pot Liner (SPL) induced DNA damage and nuclear alterations in root tip cells of Allium cepa as a consequence of programmed cell death
dc.typeArtigo


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