Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro

dc.creatorMüller, Alexandra
dc.creatorSchneider, Jannis F.
dc.creatorDegrossoli, Adriana
dc.creatorLupilova, Nataliya
dc.creatorDick, Tobias P.
dc.creatorLeichert, Lars I.
dc.date2018-05-25T11:22:15Z
dc.date2018-05-25T11:22:15Z
dc.date2017-04
dc.date.accessioned2023-09-28T19:54:31Z
dc.date.available2023-09-28T19:54:31Z
dc.identifierMÜLLER, A. et al. Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro. Data in Brief, [S.l.], v. 11, p. 617-627, Apr. 2017.
dc.identifierhttp://repositorio.ufla.br/jspui/handle/1/29313
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9039863
dc.descriptionThis article contains representative fluorescence excitation spectra of roGFP2-based probes used for ratiometric analysis of redox changes as presented in the article "Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species". The recombinant probes roGFP2, roGFP2-Orp1, and Grx1-roGFP2 were exposed to various oxidative and nitrosative species, including hydrogen peroxide (H2O2), aldrithiol-2 (AT-2), glutathione disulfide (GSSG), hypochlorous acid (HOCl), S-nitrosoglutathione (GSNO), peroxynitrite (ONOO−), potassium polysulfide (K2Sx), spermine NONOate (SperNO), and diethyl amino NONOate (DeaNO) at different molar ratios. Fluorescence excitation spectra of the probes were recorded in the excitation wavelength range between 350 and 500 nm and for a total of 60 min. Analysis and interpretation of the data is presented in an associated article.
dc.formatapplication/pdf
dc.languageen_US
dc.publisherElsevier
dc.rightsacesso aberto
dc.rightshttp://creativecommons.org/licenses/by/4.0/
dc.sourceData in Brief
dc.subjectGenetically encoded redox probes
dc.subjectPolysulfides
dc.subjectHypochlorous acid (HOCl)
dc.subjectPeroxynitrite
dc.subjectGlutathione
dc.subjectHydrogen peroxide (H2O2)
dc.subjectNitricoxide
dc.titleFluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro
dc.typeArtigo


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