Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic

dc.creatorLIMA, Eleonidas Moura
dc.creatorLOPES, Otávio Sérgio
dc.creatorSOARES, Leonardo Ferreira
dc.creatorARRUDA, Talitta Dantas
dc.creatorGIGEK, Carolina Oliveira
dc.creatorMELO, Cynthia Germoglio Farias
dc.creatorSMITH, Marilia Arruda Cardoso
dc.creatorOLIVEIRA, João Ricardo Gonçalves
dc.creatorMEDEIROS, Arnaldo Correia de
dc.creatorDELATORRE, Plínio
dc.creatorRODRÍGUEZ BURBANO, Rommel Mario
dc.date2017-04-06T17:17:25Z
dc.date2017-04-06T17:17:25Z
dc.date2015-06
dc.date.accessioned2023-09-28T15:50:20Z
dc.date.available2023-09-28T15:50:20Z
dc.identifierLIMA, Eleonidas Moura et al. Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic. Brazilian Archives of Biology and Technology, Curitiba, v. 58, n. 3, p. 414-420, mai./jun. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300414&lng=pt&nrm=iso>. Acesso em: 06 abr. 2017. <http://dx.doi.org/10.1590/S1516-8913201500434>.
dc.identifier1678-4324
dc.identifierhttp://repositorio.ufpa.br/jspui/handle/2011/8103
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9022125
dc.descriptionGastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ 2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ 2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination.
dc.formatapplication/pdf
dc.languagepor
dc.publisherUniversidade Federal do Pará
dc.publisherBrasil
dc.publisherUFPA
dc.relationBrazilian Archives of Biology and Technology
dc.rightsAcesso Aberto
dc.subjectCâncer gástrico
dc.subjectGenótipo
dc.subjectPolimofismo de nucleotídeo
dc.subjectOncogenética
dc.subjectCarcinoma gástrico
dc.subjectCNPQ::CIENCIAS EXATAS E DA TERRA
dc.titleDideoxy single allele-specific PCR - DSASP new method to discrimination allelic
dc.typeArtigo de Periódico


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