Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon

dc.creatorDALL'AGNOL, Leonardo Teixeira
dc.creatorMARTINS, Rosimar Neris
dc.creatorVALLINOTO, Antonio Carlos Rosário
dc.creatorRIBEIRO, Karla Tereza Silva
dc.date2013-04-02T12:58:21Z
dc.date2013-04-02T12:58:21Z
dc.date2008-11
dc.date.accessioned2023-09-28T15:44:13Z
dc.date.available2023-09-28T15:44:13Z
dc.identifierDALL'AGNOL, L. T., et al. Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 7, p. 678-682, nov. 2008. Disponível em: <http://www.scielo.br/pdf/mioc/v103n7/09.pdf>. Acesso em: 01 abr. 2013. <http://dx.doi.org/10.1590/S0074-02762008000700009>.
dc.identifier0074-0276
dc.identifierhttp://repositorio.ufpa.br/jspui/handle/2011/3611
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9020226
dc.descriptionThe present study intended to characterize the phenotypic and genetic diversity of Brazilian isolates of Chromobacterium violaceum from aquatic environments within the Amazon region. Nineteen isolates showed morphological properties of C. violaceum and the majority grew at 44°C. Low temperatures, in contrast, showed to be inhibitory to their growth, as eleven isolates did not grow at 10ºC and nine did not produce pigmentation, clearly indicating an inhibition of their metabolism. The largest variation among isolates was observed in the citrate test (Simmons), in which 12 isolates were positive, and in the oxidation/fermentation of sucrose, with six positives isolates. Chloramphenicol, gentamicin and sulfonamides efficiently inhibited bacterial growth. Amplified products of the recA gene were digested with HindII or PstI, which produced three or four restriction fragments patterns, respectively. The combined analysis arranged the isolates into six genospecies. The higher diversity observed in Belém (genotypes C, D, E and F) may be a consequence of intense human occupation, pollution of the aquatic environment or due to the higher diversity of the environments sampled in that region. In conclusion, a high level of genetic and phenotypic diversity was observed, and four new genospecies were described.
dc.formatapplication/pdf
dc.languageeng
dc.rightsAcesso Aberto
dc.subjectAmbiente aquático
dc.subjectChromobacterium violaceum
dc.subjectVariação genética
dc.subjectAmazônia brasileira
dc.titleDiversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon
dc.typeArtigo de Periódico


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