Mucoadhesive gellan gum-based and carboxymethyl cellulose -based hydrogels containing gemcitabine and papain for bladder cancer treatment

dc.creatorLIMA, CAROLINE S.A. de
dc.creatorRIAL-HERMIDA, MARIA I.
dc.creatorFREITAS, LUCAS F. de
dc.creatorPEREIRA-DA-MOTA, ANA F.
dc.creatorVIVERO-LOPEZ, MARIA
dc.creatorFERREIRA, ARYEL H.
dc.creatorKAD??UBOWSKI, S??AWOMIR
dc.creatorVARCA, GUSTAVO H.C.
dc.creatorLUGAO, ADEMAR B.
dc.creatorALVAREZ-LORENZO, CARMEN
dc.date2023
dc.date2023-06-29T12:35:31Z
dc.date2023-06-29T12:35:31Z
dc.date.accessioned2023-09-28T14:26:12Z
dc.date.available2023-09-28T14:26:12Z
dc.identifier0141-8130
dc.identifierhttp://repositorio.ipen.br/handle/123456789/34103
dc.identifier3
dc.identifier242
dc.identifier10.1016/j.ijbiomac.2023.124957
dc.identifier0000-0002-1737-3191
dc.identifier91.2
dc.identifier92.67
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9004311
dc.descriptionLocal treatment of bladder cancer faces several limitations such as short residence time or low permeation through urothelium tissue. The aim of this work was to develop patient-friendly mucoadhesive gel formulations combining gemcitabine and the enzyme papain for improved intravesical chemotherapy delivery. Hydrogels based on two different polysaccharides, gellan gum and sodium carboxymethylcellulose (CMC), were prepared with either native papain or papain nanoparticles (nanopapain) to explore for the first time their use as permeability enhancers through bladder tissue. Gel formulations were characterized regarding enzyme stability, rheological behavior, retention on bladder tissue and bioadhesion, drug release properties, permeation capacity, and biocompatibility. After 90 days of storage, the enzyme loaded in the CMC gels retained up to 83.5 ?? 4.9 % of its activity in the absence of the drug, and up to 78.1 ?? 5.3 with gemcitabine. The gels were mucoadhesive and the enzyme papain showed mucolytic action, which resulted in resistance against washing off from the urothelium and enhanced permeability of gemcitabine in the ex vivo tissue diffusion tests. Native papain shortened lag-time tissue penetration to 0.6 h and enhanced 2-fold drug permeability All formulations demonstrated pseudoplastic behavior and no irritability. Overall, the developed formulations have potential as an upgraded alternative to intravesical therapy for bladder cancer treatment.
dc.descriptionFunda????o de Amparo ?? Pesquisa do Estado de S??o Paulo (FAPESP)
dc.descriptionSpain, Ministry for Science and Innovation (MCIN)
dc.descriptionXunta de Galicia
dc.descriptionFAPESP: 17/50332???0
dc.descriptionMCIN: 2020???1184 113881RB-I00/AEI/10.130 39/501100011033
dc.descriptionXunta de Galicia: ED431C 2020/17
dc.format1-16
dc.relationInternational Journal of Biological Macromolecules
dc.rightsopenAccess
dc.subjecthydrogels
dc.subjectpapain
dc.subjectpolysaccharides
dc.subjectdrug delivery
dc.subjectbladder
dc.subjectneoplasms
dc.titleMucoadhesive gellan gum-based and carboxymethyl cellulose -based hydrogels containing gemcitabine and papain for bladder cancer treatment
dc.typeArtigo de peri??dico
dc.coverageI


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