Sandwich-like electrochemical aptasensing of heat shock protein 70 kDa (HSP70)

dc.creatorNEGAHDARY, MASOUD
dc.creatorHIRATA, MARIO H.
dc.creatorSAKATA, SOLANGE K.
dc.creatorCICONELLI, ROZANA M.
dc.creatorBASTOS, GISELE M.
dc.creatorBORGES, JESSICA B.
dc.creatorTHUROW, HELENA S.
dc.creatorSILVEIRA JUNIOR, ALCEU T.
dc.creatorSAMPAIO, MARCELO F.
dc.creatorGUIMARAES, LARISSA B.
dc.creatorMAEDA, BRUNO S.
dc.creatorANGNES, LUCIO
dc.date2023
dc.date2023-04-14T15:49:56Z
dc.date2023-04-14T15:49:56Z
dc.date.accessioned2023-09-28T14:25:43Z
dc.date.available2023-09-28T14:25:43Z
dc.identifier0003-2670
dc.identifierhttp://repositorio.ipen.br/handle/123456789/33975
dc.identifier1242
dc.identifier10.1016/j.aca.2022.340716
dc.identifier0000-0001-6072-5853
dc.identifier91.3
dc.identifier87.75
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9004184
dc.descriptionIn this research, by using aptamer-conjugated gold nanoparticles (aptamer-AuNPs) and a modified glassy carbon electrode (GCE) with reduced graphene oxide (rGO) and Acropora-like gold (ALG) nanostructure, a sandwich-like system provided for sensitive detection of heat shock protein 70 kDa (HSP70), which applied as a functional biomarker in diagnosis/prognosis of COVID-19. Initially, the surface of the GCE was improved with rGO and ALG nanostructures, respectively. Then, an aptamer sequence as the first part of the bioreceptor was covalently bound on the surface of the GCE/rGO/ALG nanostructures. After adding the analyte, the second part of the bioreceptor (aptamer-AuNPs) was immobilized on the electrode surface to improve the diagnostic performance. The designed aptasensor detected HSP70 in a wide linear range, from 5 pg mL???1 to 75 ng mL???1, with a limit of detection (LOD) of ???2 pg mL???1. The aptasensor was stable for 3 weeks and applicable in detecting 40 real plasma samples of COVID-19 patients. The diagnostic sensitivity and specificity were 90% and 85%, respectively, compared with the reverse transcription-polymerase chain reaction (RT-PCR) method.
dc.descriptionFunda????o de Amparo ?? Pesquisa do Estado de S??o Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico (CNPq)
dc.descriptionFAPESP: 19/27021-4; 17/13137-5; 14/50867-3
dc.descriptionCNPq: 311847/2018-8; 465389/2014-7
dc.format1-10
dc.relationAnalytica Chimica Acta
dc.rightsopenAccess
dc.subjectgold
dc.subjectnanomaterials
dc.subjectcoronaviruses
dc.subjectelectrochemistry
dc.subjectelectrodes
dc.subjectgraphene
dc.titleSandwich-like electrochemical aptasensing of heat shock protein 70 kDa (HSP70)
dc.typeArtigo de peri??dico
dc.coverageI


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