| dc.creator | TEIXEIRA, LUIZ F.S. | |
| dc.creator | BELLINI, MARIA H. | |
| dc.creator | ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY (SBBq), 51st; CONGRESS OF BRAZILIAN BIOPHYSICAL SOCIETY (SBBf)/LATIN AMERICAN FEDERATION OF BIOPHYSICAL SOCIETIES (Lafebs), 46th | |
| dc.date | 2023-03-21T14:50:26Z | |
| dc.date | 2023-03-21T14:50:26Z | |
| dc.date | September 5-8, 2022 | |
| dc.date.accessioned | 2023-09-28T14:25:30Z | |
| dc.date.available | 2023-09-28T14:25:30Z | |
| dc.identifier | http://repositorio.ipen.br/handle/123456789/33907 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/9004126 | |
| dc.description | INTRODUCTION: Renal cell carcinoma (RCC) is the most common adult renal epithelial cancer. The most frequent
subtype of RCC is clear cell (ccRCC). Most of ccRCC patients have a mutation in the Von Hippel-Lindau (VHL) tumor
suppressor gene. The VHL gene encodes a protein, the VHL, which can up-regulate a series of intracellular proteins,
including the hypoxia inducible factor (HIF). The transcription factor NF-??B is increased in the ccRCC. OBJECTIVES:
To evaluate the impact of the NF-??B1 gene knockout on the VEGF and IL6 expression in the human RCC cells under
normoxia and hypoxia. MATERIALS AND METHODS: The CRISPR/Cas-9 technique was used to obtain 786-0 cells
knockout for the NF-??B1 protein. Western Blot assay was used to selected the clones. A hypoxia-inducing humid
chamber was used and its effectiveness was validated its effectiveness was certified by the analysis of HIF-2??
expression levels. The quantification of VEGF and IL-6 levels was measured using Real Time-PCR and MILLIPLEX
assay. DISCUSSION AND RESULTS: The VEGF gene expression in the clones was significantly lower than that
presented by the control both in normoxia (786-0-sg1 99.68??0.09%, 786-0-sg2 78.55??0.85%, 786-0-sg3
91.70??0.87%) and in hypoxia (786-0-sg1 98.30??1.49%, 786-0-sg2 75.21??4.14%, 786-0-sg3 98.44??0.18%). The
expression of IL-6 gene was also significant lower in normoxia (786-0-sg1 49.03??0.80%, 786-0-sg2 76.59??12.43%,
786-0-sg3 66.98??10.89%) and in hypoxia (786-0-sg1 95.85??0.36%, 786-0-sg2 96.45??0.49%, 786-0-sg3
91.08??1.42%). The MILLIPLEX results show that there was a significant reduction of both VEGF and IL-6 in the culture
medium of cells knocked out in normoxia and hypoxia compared to control group. CONCLUSION: Suppression of p50
expression in the clones resulted in the reduction of VEGF and IL6 in both conditions. The reduction in the IL-6 relative
expression hypoxia/normoxia demonstrates a change in cellular responsiveness to decreased levels of oxygen. | |
| dc.format | 415-415 | |
| dc.publisher | Sociedade Brasileira de Bioqu??mica e Biologia Molecular - SBBq | |
| dc.rights | openAccess | |
| dc.title | Evaluation of angiogenic capacity of human adenocarcinoma cell line knockout for NF-????1 protein | |
| dc.type | Resumo de eventos cient??ficos | |
| dc.coverage | I | |
| dc.local | S??o Paulo, SP | |
