dc.creatorCONTATORI, CAROLINA G. de S.
dc.creatorPINTO, MAYARA S.
dc.creatorRIBEIRO, MARTHA S.
dc.creatorCONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th
dc.date2022-03-25T17:47:20Z
dc.date2022-03-25T17:47:20Z
dc.dateOctober 4-8, 2021
dc.date.accessioned2023-09-28T14:21:38Z
dc.date.available2023-09-28T14:21:38Z
dc.identifierhttp://repositorio.ipen.br/handle/123456789/32869
dc.identifier0000-0002-4203-1134
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9003088
dc.descriptionCell migration plays an important role in tissue formation and cancer progression. In vitro scratch assay has been used for many years to study cell migration to mimic the migration of in vivo cells, and, thus, to evaluate cancer growth. Low-level red and near-infrared light (LLL) can increase normal cell migration. However, the impact of LLL on tumor cells remains unclear. In this work, we aimed to evaluate the effects of a single LLL dose on melanoma cell migration. B16F10 (murine melanoma) cells were cultivated in RPMI medium with 10% of fetal bovine serum until they reached 80% confluency. The cell line was seeded in a 6-well plate at a density of 2x10 5 cells/well in triplicate at two different moments. A wound scratch was performed to disrupt the confluent cell monolayer with a 10 ??L pipette tip. Immediately after the injury, the cells were submitted to the LLL at two distinct wavelengths (660 and 780 nm) provided by a LED and a laser, respectively, delivering 3 different energies (1.3, 3.6, and 6 J) at an irradiance of 4.2 mW/cm2. The control group was not irradiated. Cells were photographed immediately and at 3, 12, 24, and 36 h after the scratch. The wound closure was measured using ImageJ software. To evaluate the overall migration, we calculated the areas under the curve for each group. Cells exposed to the red laser at 6 J migrated slower than control. In contrast, LLL at 780 nm promoted faster cell migration when irradiated with 3.6 J. These results suggest that low-level LEDs at 660 nm could prevent melanoma progression in higher energies. However, 780 nm should be avoided at middle energies.
dc.format249-249
dc.publisherSociedade Brasileira de Bioqu??mica e Biologia Molecular (SBBq)
dc.rightsopenAccess
dc.subjectmelanomas
dc.subjectmodulation
dc.subjectinfrared radiation
dc.subjectlight sources
dc.subjectcell proliferation
dc.subjectmigration
dc.subjectin vitro
dc.titleMelanoma cell migration in response to red and near-infrared low-level light
dc.typeResumo de eventos cient??ficos
dc.coverageI
dc.localS??o Paulo, SP


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