Rational selection of hidden epitopes for a molecularly imprinted electrochemical sensor in the recognition of heat-denatured dengue NS1 protein

dc.creatorSILVA, MATHEUS S.
dc.creatorTAVARES, ANA P.M.
dc.creatorCOELHO, LUIZ F.L.
dc.creatorDIAS, LIGIA E.M.F.
dc.creatorCHURA-CHAMBI, ROSA M.
dc.creatorFONSECA, FLAVIO G. da
dc.creatorSALES, MARIA G.F.
dc.creatorFIGUEIREDO, EDUARDO C.
dc.date2021
dc.date2022-01-25T12:02:52Z
dc.date2022-01-25T12:02:52Z
dc.date.accessioned2023-09-28T14:20:47Z
dc.date.available2023-09-28T14:20:47Z
dc.identifier0956-5663
dc.identifierhttp://repositorio.ipen.br/handle/123456789/32642
dc.identifier191
dc.identifier10.1016/j.bios.2021.113419
dc.identifier93.02
dc.identifier97.00
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9002861
dc.descriptionRational selection of predicted peptides to be employed as templates in molecular imprinting was carried out for the heat-denatured non-structural protein 1 (NS1) of dengue virus (DENV). Conservation analysis among 301 sequences of Brazilian isolates of DENV and zika virus (ZIKV) NS1 was carried out by UniProtKB, and peptide selection was based on in silico data of the conservational, structural and immunogenic properties of the sequences. The selected peptide (from dengue 1 NS1) was synthesized and employed as a template in the electropolymerization of polyaminophenol-imprinted films on the surface of carbon screen-printed electrodes. Heat denaturation of the protein was carried out prior to analysis, in order to expose its internal hidden epitopes. After removal of the template, the molecularly imprinted cavities were able to rebind to the whole denatured protein as determined by electrochemical impedance spectroscopy. This label-free sensor was efficient to distinguish the NS1 of DENV from the NS1 of ZIKV. Additionally, the sensor was also selective for dengue NS1, in comparison with human serum immunoglobulin G and human serum albumin. Additionally, the device was able to detect the DENV NS1 at concentrations from 50 to 200 ??g L???1 (RSD below 5.04%, r = 0.9678) in diluted human serum samples. The calculated LOD and LOQ were, respectively, 29.3 and 88.7 ??g L???1 and each sensor could be used for six sequential cycles with the same performance.
dc.descriptionFunda????o de Amparo ?? Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.descriptionConselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico (CNPq)
dc.descriptionCoordena????o de Aperfei??oamento de Pessoal de N??vel Superior (CAPES)
dc.descriptionFAPEMIG: CDS-APQ-00638-17
dc.descriptionCNPq: 301564/2019-1; 427365/2018-0
dc.descriptionCAPES: 88881.133746/2016-01
dc.format1-8
dc.relationBiosensors and Bioelectronics
dc.rightsopenAccess
dc.subjectviral diseases
dc.subjectviral diseases
dc.subjectzika virus
dc.subjectprotein denaturation
dc.subjectpolymers
dc.subjectsensors
dc.titleRational selection of hidden epitopes for a molecularly imprinted electrochemical sensor in the recognition of heat-denatured dengue NS1 protein
dc.typeArtigo de peri??dico
dc.coverageI


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