Disposable electrochemical microfluidic device for ultrasensitive detection of egg allergen in wine samples

dc.creatorBALDO, THAISA A.
dc.creatorPROENCA, CAMILA dos A.
dc.creatorFELIX, FABIANA da S.
dc.creatorFREITAS, TAYANE A.
dc.creatorSAKATA, SOLANGE K.
dc.creatorANGNES, LUCIO
dc.creatorFARIA, RONALDO C.
dc.date2021
dc.date2021-12-13T13:36:36Z
dc.date2021-12-13T13:36:36Z
dc.date.accessioned2023-09-28T14:19:54Z
dc.date.available2023-09-28T14:19:54Z
dc.identifier0039-9140
dc.identifierhttp://repositorio.ipen.br/handle/123456789/32396
dc.identifier232
dc.identifier10.1016/j.talanta.2021.122447
dc.identifier0000-0001-6072-5853
dc.identifier87.93
dc.identifier89.00
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9002616
dc.descriptionFood allergies have been increasing all over the world. Egg is an important component in the food industries and the second most common cause of food allergy, shortly after milk. In the wine industry, egg white is applied as a fining agent for tannin removal. In this study, a sandwich-based immunoassay for ultrasensitive detection of ovalbumin (OVA) in wine samples was developed. The assay involves the use of magnetic beads (MBs) decorated with a polyclonal anti-OVA antibody (Ab2) and horseradish peroxidase (HRP), used as label for the quantification in a disposable electrochemical microfluidic device (DE??D) here developed. The Ab2-MB-HRP prepared was applied to capture, separate, and pre-concentrate OVA from wine samples. In the DE??D, OVA was immune-magnetically captured (OVA-Ab2-MB-HRP), producing a sandwich structure (GO-Ab1-OVA-Ab2-MB-HRP) on the electrode's surface. This arrangement results in an ultrasensitive device, achieving the ultralow limit of detection of 0.2 fg mL???1 OVA. Five samples of wines were analyzed by using the immuno-magneto-assay which presents excellent accuracy compared with enzyme-linked immunosorbent assay (ELISA).
dc.descriptionCoordena????o de Aperfei??oamento de Pessoal de N??vel Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico (CNPq)
dc.descriptionFunda????o de Amparo ?? Pesquisa do Estado de S??o Paulo (FAPESP)
dc.descriptionCAPES: 001
dc.descriptionCNPq: 308570/2018-9; 311847/2018-8
dc.descriptionFAPESP: 13/14262-7; 16/00991-5; 17/24053-7; 17/13137-5; 14/50867-3
dc.format1-9
dc.relationTalanta
dc.rightsopenAccess
dc.subjectegg
dc.subjectallergy
dc.subjectovalbumin
dc.subjectenzyme immunoassay
dc.subjectbeverages
dc.subjectantigens
dc.titleDisposable electrochemical microfluidic device for ultrasensitive detection of egg allergen in wine samples
dc.typeArtigo de peri??dico
dc.coverageI


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