A mechanistic approach towards the formation of bityrosine in proteins by ionizing radiation

dc.creatorSOWINSKI, SEBASTIAN
dc.creatorVARCA, GUSTAVO H.C.
dc.creatorKADLUBOWSKI, SLAWOMIR
dc.creatorLUGAO, ADEMAR B.
dc.creatorULANSKI, PIOTR
dc.date2021
dc.date2021-12-10T14:04:50Z
dc.date2021-12-10T14:04:50Z
dc.date.accessioned2023-09-28T14:19:52Z
dc.date.available2023-09-28T14:19:52Z
dc.identifier0969-806X
dc.identifierhttp://repositorio.ipen.br/handle/123456789/32383
dc.identifier188
dc.identifier10.1016/j.radphyschem.2021.109644
dc.identifier0000-0002-1737-3191
dc.identifier0000-0002-6545-237X
dc.identifier57.09
dc.identifier70.00
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9002603
dc.descriptionOne of the methods of protein crosslinking used in the synthesis of protein-based nanoparticles is the formation of bityrosine bridges, which may be achieved by the recombination of phenoxyl-type radicals in irradiated protein solutions. Radiation-induced formation of phenoxyl radicals in tyrosine is promoted in presence of H+. In this work, kinetics of this process and the influence of pH were studied by pulse radiolysis measurements of a model peptide H-Gly-Tyr-Gly-OH (GYG), which may help to solve questions raised for bigger, more complex systems in comparable conditions. The main route of phenoxyl radicals formation consists of the addition reaction of hydroxyl radical to the phenol ring on the tyrosine side-chain and proton catalyzed water molecule elimination. A similar effect was observed in studies of tripeptide solutions containing phosphate buffer. The presented data include time-resolved optical spectroscopy of transient species formed under pulse electron beam irradiation and a comparison of the kinetics of the phenoxyl radicals formation in samples at various pH and in presence of phosphate buffer. The postulated mechanism and obtained values of rate constants of the formation and decay of transient species were additionally checked by simple probabilistic simulations.
dc.descriptionFunda????o de Amparo ?? Pesquisa do Estado de S??o Paulo (FAPESP)
dc.descriptionInternational Atomic Energy Agency (IAEA)
dc.descriptionConselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico (CNPq)
dc.descriptionFAPESP: 10/10935-9; 15/13979-0
dc.descriptionIAEA: CRP F22064
dc.descriptionCNPq: 402887/2013-1
dc.format1-8
dc.relationRadiation Physics and Chemistry
dc.rightsopenAccess
dc.subjectionizing radiations
dc.subjectradiolysis
dc.subjecttyrosine
dc.subjectproteins
dc.titleA mechanistic approach towards the formation of bityrosine in proteins by ionizing radiation
dc.typeArtigo de peri??dico
dc.coverageI


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