Semi-solid pharmaceutical formulations for the delivery of papain nanoparticles

dc.creatorLIMA, CAROLINE S.A. de
dc.creatorVARCA, JUSTINE P.R.O.
dc.creatorNOGUEIRA, KAMILA M.
dc.creatorFAZOLIN, GABRIELA N.
dc.creatorFREITAS, LUCAS F. de
dc.creatorSOUZA, ELISEU W. de
dc.creatorLUGAO, ADEMAR B.
dc.creatorVARCA, GUSTAVO H.C.
dc.date2020
dc.date2021-02-24T14:52:56Z
dc.date2021-02-24T14:52:56Z
dc.date.accessioned2023-09-28T14:17:49Z
dc.date.available2023-09-28T14:17:49Z
dc.identifier1999-4923
dc.identifierhttp://repositorio.ipen.br/handle/123456789/31810
dc.identifier12
dc.identifier12
dc.identifier10.3390/pharmaceutics12121170
dc.identifier0000-0002-6545-237X
dc.identifier0000-0002-1737-3191
dc.identifier89.64
dc.identifier68.00
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9002033
dc.descriptionPapain is a therapeutic enzyme with restricted applications due to associated allergenic reactions. Papain nanoparticles have shown to be safe for biomedical use, although a method for proper drug loading and release remains to be developed. Thus, the objective of this work was to develop and assess the stability of papain nanoparticles in a prototype semi-solid formulation suitable for dermatological or topical administrations. Papain nanoparticles of 7.0 ?? 0.1 nm were synthesized and loaded into carboxymethylcellulose- and poly(vinyl alcohol)-based gels. The formulations were then assayed for preliminary stability, enzyme activity, cytotoxicity studies, and characterized according to their microstructures and protein distribution. The formulations were suitable for papain nanoparticle loading and provided a stable environment for the nanoparticles. The enzyme distribution along the gel matrix was homogeneous for all the formulations, and the proteolytic activity was preserved after the gel preparation. Both gels presented a slow release of the papain nanoparticles for four days. Cell viability assays revealed no potential cytotoxicity, and the presence of the nanoparticles did not alter the microstructure of the gel. The developed systems presented a potential for biomedical applications, either as drug delivery systems for papain nanoparticles and/or its complexes.
dc.descriptionFunda????o de Amparo ?? Pesquisa do Estado de S??o Paulo (FAPESP)
dc.descriptionInternational Atomic Energy Agency (IAEA)
dc.descriptionConselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico (CNPq)
dc.descriptionSistema Nacional de Laborat??rios em Nanotecnologias (SisNANO)
dc.descriptionFAPESP: 19/01315-1; 18/151598-2; 15/13979-0
dc.descriptionIAEA: CRP F22064
dc.descriptionCNPq: 402887/2013-1
dc.descriptionSisNano: 442527/2019-5
dc.format1-12
dc.relationPharmaceutics
dc.rightsopenAccess
dc.subjectpapain
dc.subjectnanoparticles
dc.subjectdrugs
dc.subjectgels
dc.subjectproteins
dc.titleSemi-solid pharmaceutical formulations for the delivery of papain nanoparticles
dc.typeArtigo de peri??dico
dc.coverageI


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