In vivo activity of silver nanoparticles against Pseudomonas aeruginosa infection in Galleria mellonella

dc.creatorTHOMAZ, LUCIANA
dc.creatorALMEIDA, LUIZ G. de
dc.creatorSILVA, FLAVIA R.O.
dc.creatorCORTEZ, MAURO
dc.creatorTABORDA, CARLOS P.
dc.creatorSPIRA, BENY
dc.date2020
dc.date2021-02-23T12:22:01Z
dc.date2021-02-23T12:22:01Z
dc.date.accessioned2023-09-28T14:17:45Z
dc.date.available2023-09-28T14:17:45Z
dc.identifier1664-302X
dc.identifierhttp://repositorio.ipen.br/handle/123456789/31791
dc.identifier11
dc.identifier10.3389/fmicb.2020.582107
dc.identifier79.93
dc.identifier81.00
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9002014
dc.descriptionPseudomonas aeruginosa is an opportunistic pathogen associated with life-threatening nosocomial and community-acquired infections. Antibiotic resistance is an immediate threat to public health and demands an urgent action to discovering new antimicrobial agents. One of the best alternatives for pre-clinical tests with animal models is the greater wax moth Galleria mellonella. Here, we evaluated the antipseudomonal activity of silver nanoparticles (AgNPs) against P. aeruginosa strain UCBPP-PA14 using G. mellonella larvae. The AgNPs were synthesized through a non-toxic biogenic process involving microorganism fermentation. The effect of AgNPs was assessed through characterization and quantification of the hemocytic response, nodulation and phenoloxidase cascade. On average, 80% of the larvae infected with P. aeruginosa and prophylactically treated with nanoparticles survived. Both the specific and total larvae hemocyte counts were restored in the treated group. In addition, the nodulation process and the phenoloxidase cascade were less exacerbated when the larvae were exposed to the silver nanoparticles. AgNPs protect the larvae from P. aeruginosa infection by directly killing the bacteria and indirectly by preventing an exacerbated immunological response against the pathogen. Our results suggest that the prophylactic use of AgNPs has a strong protective activity against P. aeruginosa infection.
dc.descriptionFunda????o de Amparo ?? Pesquisa do Estado de S??o Paulo (FAPESP)
dc.descriptionFAPESP: 16/08730-6; 12/24105-3; 11/07775-2; 15/06547-7; 14/09143-1
dc.format1-14
dc.relationFrontiers in Microbiology
dc.rightsopenAccess
dc.subjectlepidoptera
dc.subjectbacteria
dc.subjectpseudomonas
dc.subjectnanoparticles
dc.subjectsilver
dc.subjectantibiotics
dc.subjectantimicrobial agents
dc.subjecttoxicity
dc.titleIn vivo activity of silver nanoparticles against Pseudomonas aeruginosa infection in Galleria mellonella
dc.typeArtigo de peri??dico
dc.coverageI


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