Avoiding chemotherapy resistance in squamous cell carcinomas

dc.creatorHERNANDES, CAMILA
dc.creatorSILVA, BRUNA da
dc.creatorKLINGBEIL, MARIA de F.G.
dc.creatorMATHOR, MONICA B.
dc.creatorPEREIRA, ANA M.S.
dc.creatorSEVERINO, PATRICIA
dc.date2017
dc.date2020-06-24T19:20:27Z
dc.date2020-06-24T19:20:27Z
dc.date.accessioned2023-09-28T14:15:59Z
dc.date.available2023-09-28T14:15:59Z
dc.identifier0008-5472
dc.identifierhttp://repositorio.ipen.br/handle/123456789/31338
dc.identifier77
dc.identifier13 Supplement
dc.identifier10.1158/1538-7445.AM2017-5456
dc.identifier0000-0002-7294-9106
dc.identifier92.601
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9001561
dc.descriptionMicroRNAs are small noncoding RNAs that play important roles in cellular biology. They have been implicated in pharmacogenomics by down-regulating genes that are essential for drug function. In this work we verified the potential anticancer activity of the quinone methide triterpenes maytenin and 22-??-hydroxymaytenin, as well as of a quinone methide triterpene-rich extract obtained from cultivated Maytenus ilicifolia root cells, and evaluated the associated microRNA expression following half maximal inhibitory concentration (IC50) treatment. Standard selectivity index (SI) for the isolated compounds and the root cell extract was determined by the logarithmic shift in effective concentration (IC50) between cancer cell lines and oral keratinocytes. Both isolated molecules as well as the root cell extract presented pronounced antiproliferative and pro-apoptotic activities in four cell lines derived from head and neck squamous cell carcinomas, including a metastasis-derived cell line. A positive SI, with an average 2-fold increase in potency, was detected for single agents and for the extract. MicroRNA expression profiles were assessed at 24h, 48h and 72h following treatment and an average of 100 molecules presented consistent marked variation in expression levels. Considering associations of microRNAs, genes they regulate, and the drugs effects dependent on these genes, the down-regulation of miR-193a-3p and miR-21 in treated cells is of particular interest. Both microRNAs have been involved in 5-fluorouracil and cisplatin resistance, current agents of standard chemoradiotherapy for locally advanced head and neck cancer. Squamous cell carcinoma of the head and neck is one of the most common cancer types worldwide whereas treatment options based on conventional therapies or targeted therapies under development have limited efficacy. Plant-derived products are valuable sources for the development of new therapeutic options for cancer treatment or as synergistic agents in existing regular care.
dc.relationCancer Research
dc.rightsopenAccess
dc.sourceAmerican Association for Cancer Research Annual Meeting, April 1-5, 2017, Washington, DC, USA
dc.subjectchemotherapy
dc.subjectneoplasms
dc.subjectepithelium
dc.subjecthistology
dc.subjectrna
dc.subjectinhibition
dc.titleAvoiding chemotherapy resistance in squamous cell carcinomas
dc.typeResumos em peri??dicos
dc.coverageI


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