Synthesis of paramagnetic iron oxide nanoparticles for application in in vitro three-dimensional biological models through electron beam irradiation and microwave reduction of iron ions

dc.creatorPASSOS, PRISCILA de Q.S.
dc.creatorCORAZZA, FULVIO G.
dc.creatorLIMA, MAYELLE M.P.
dc.creatorTOMINAGA, FLAVIO K.
dc.creatorSAKATA, SOLANGE K.
dc.creatorGON??ALVES, KARINA O.
dc.creatorCOURROL, LILIA C.
dc.creatorVIEIRA, DANIEL P.
dc.creatorINTERNATIONAL NUCLEAR ATLANTIC CONFERENCE
dc.date2020-01-10T17:17:09Z
dc.date2020-01-10T17:17:09Z
dc.dateOctober 21-25, 2019
dc.date.accessioned2023-09-28T14:13:27Z
dc.date.available2023-09-28T14:13:27Z
dc.identifierhttp://repositorio.ipen.br/handle/123456789/30624
dc.identifier0000-0002-0007-534X
dc.identifier0000-0001-6072-5853
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9000852
dc.descriptionThree-dimensional (3D) cell culture is increasingly being used in assays to assess the safety and efficacy of new drug candidates. Tumor cell spheroids can mimic with high precision the biological complexity of cellular interactions with their tumor microenvironment. Currently, several techniques can be used to construct 3D spheroids. Among them, magnetic levitation is one of the most used in biomedical research. This technique consists in the magnetization of cells through the adsorption of magnetic nanoparticles of iron oxide (Fe3O4) that are produced by the reaction of Fe2+ and Fe3+ ions in alkaline medium. In this work, nanoparticles of paramagnetic iron oxide (PIONS) were synthesized by coprecipitation through electron beam irradiation at 15 and 30 kGy doses. After functionalization with polar amino acids, nanoparticle suspensions were characterized by physical-chemical assays that showed the successful attachment of the carboxylate groups to the iron, explaining the ability of the particles to adsorb the membranes. Cytotoxicity assay showed that the nanoparticles synthesized by microwave (MW) and electron beam had no toxicity. Others biological assays have also shown efficient adsorption of the particles by human prostate tumor cells, allowing the in vitro application of a biomimetic 3D biological model with potential utilization regarding the development and evaluation of antitumor drugs and radiopharmaceuticals for the treatment of prostate cancer.
dc.format2296-2309
dc.publisherAssocia????o Brasileira de Energia Nuclear
dc.rightsopenAccess
dc.subjectbiological models
dc.subjectdrugs
dc.subjectelectron beams
dc.subjectin vitro
dc.subjectiron ions
dc.subjectiron oxides
dc.subjectirradiation
dc.subjectmicrowave radiation
dc.subjectnanoparticles
dc.subjectneoplasms
dc.subjectprostate
dc.subjectradiopharmaceuticals
dc.subjecttherapy
dc.titleSynthesis of paramagnetic iron oxide nanoparticles for application in in vitro three-dimensional biological models through electron beam irradiation and microwave reduction of iron ions
dc.typeTexto completo de evento
dc.coverageI
dc.localRio de Janeiro


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