Use of 2-color flow cytometry to assess radiation-induced genotoxic damage on CHO-KI cells

dc.creatorCARVALHO, LUMA R. de
dc.creatorBONFIM, LETICIA
dc.creatorVIEIRA, DANIEL P.
dc.date2019
dc.date2019-08-09T11:04:01Z
dc.date2019-08-09T11:04:01Z
dc.date.accessioned2023-09-28T14:11:25Z
dc.date.available2023-09-28T14:11:25Z
dc.identifier2319-0612
dc.identifierhttp://repositorio.ipen.br/handle/123456789/30049
dc.identifier2A
dc.identifier7
dc.identifier10.15392/bjrs.v7i2A.570
dc.identifier0000-0002-0007-534X
dc.identifierSem Percentil
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9000287
dc.descriptionThe micronucleus assay is an important technique used to evaluate genotoxic damage of chemical or physical agents (as ionizing radiations) on cells, based on quantification of cells bearing micronuclei, which are fragments derived from damage (breakage) of the DNA. Currently, this technique was updated to an automated approach that relies on plasma membrane dissolution to analyze fluorescent dye-labelled nuclei and micronuclei by flow cytometry. Cell suspensions were irradiated in PBS by a 60Co source in doses between 0 and 16 Gy, and incubat-ed by 72h. Cell membranes were lysed in the presence of SYTOX Green and EMA dyes, so EMA-stained nuclei could be discriminated as from dead cells, and nuclei and micronuclei could be quantified. Amounts of micronu-clei (percent of events) in the samples, were found to be proportional to radiation doses, and could be fitted to a linear-quadratic model (R?? = 0.993). Only higher doses (8 and 16 Gy) and positive control could induce relevant increases in micronucleus amounts. The incorporation EMA showed an increase in irradiated cells. Mid- to high doses (4, 8 and 16 Gy) induced reduction of cell proliferation. Experiments showed the suitability of the tech-nique to replace traditional microscopy analysis in evaluation of the effects of ionizing radiations on cells, with possibility to use in biological dosimetry.
dc.descriptionCoordena????o de Aperfei??oamento de Pessoal de N??vel Superior (CAPES)
dc.descriptionCAPES: PROEX 0041041
dc.format1-13
dc.relationBrazilian Journal of Radiation Sciences
dc.rightsopenAccess
dc.sourceThe Meeting on Nuclear Applications (ENAN), 13th, 22-27 de outubro, 2017, Belo Horizonte, MG
dc.subjectcell flow systems
dc.subjectcho cells
dc.subjectcell proliferation
dc.subjectcobalt 60
dc.subjectcell membranes
dc.subjectdosimetry
dc.subjectradiation doses
dc.subjectradiation effects
dc.subjectcell nuclei
dc.subjectgamma radiation
dc.titleUse of 2-color flow cytometry to assess radiation-induced genotoxic damage on CHO-KI cells
dc.typeArtigo de peri??dico
dc.coverageN


Este ítem pertenece a la siguiente institución