Flow cytometry based micronucleus assay for evaluation of genotoxic potential of 2-ACBs in hepatic cells HepG2

dc.creatorBARBEZAN, ANGELICA B.
dc.creatorSANTOS, CARLA J.B.
dc.creatorCARVALHO, LUMA R.
dc.creatorVIEIRA, DANIEL P.
dc.creatorSANTELLI, GLAUCIA M.M.
dc.creatorVILLAVICENCIO, ANNA L.C.H.
dc.date2019
dc.date2019-07-31T13:19:24Z
dc.date2019-07-31T13:19:24Z
dc.date.accessioned2023-09-28T14:11:11Z
dc.date.available2023-09-28T14:11:11Z
dc.identifier2319-0612
dc.identifierhttp://repositorio.ipen.br/handle/123456789/29987
dc.identifier2A
dc.identifier7
dc.identifier10.15392/bjrs.v7i2A.684
dc.identifier0000-0001-6199-7877
dc.identifier0000-0002-0007-534X
dc.identifierSem Percentil
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9000225
dc.descriptionFood irradiation is approved for use in more than 60 countries for applications and purposes in a wide variety of foods, being an effective and safe method for preservation and long-term storage. 2- Alkylcyclobutanones (2-ACBs) are the only known radiolytic products generated from foods that contain fatty acids (Triglycerides) when irradiated. The acids analyzed in this study are palmitic and stearic, which when irradiated form 2-Dodecylcyclobutanones (2-dDCB) and 2-Tetradecylcyclobutanone (2-tDCB). Part of the 2-ACBs ingested is excreted through feces and part is deposited in adipose tissues. In vitro studies so far have been only in colon cells. The work used a human hepatoma cell line (HepG2) since the accumulation of fat in this organ is quite common. Micronucleus test was selected to evaluate possible genotoxic effects of 2-dDCB and 2-tDCB compounds when exposed to high concentrations (447, 1422 and 2235 ??M) for 4 and 24 hours. Tests were performed in quadriplicates using flow cytometric analysis. None detectable genotoxic damage was observed after 4 hours of exposure to the compounds, and cytotoxic effects were only significant at the highest concentration (2235 ??M) of 2-dDCB. After 24 hours of exposure, slight genotoxic damage was observed at all concentrations evaluated, and cytotoxic effects were only present when exposed to compound 2-tDCB. Although there is a genotoxic and cytotoxic effect in some of the situations tested, the two compounds predominantly induced proliferation reduction effects of this hepatic tumor cell line.
dc.format1-24
dc.relationBrazilian Journal of Radiation Sciences
dc.rightsopenAccess
dc.sourceThe Meeting on Nuclear Applications (ENAN), 13th, 22-27 de outubro, 2017, Belo Horizonte, MG
dc.subjectfood processing
dc.subjectirradiation
dc.subjecthexadecanoic acid
dc.subjectcell flow systems
dc.subjectdodecyl radicals
dc.subjecthepatomas
dc.subjectin vitro
dc.subjectoctadecanoic acid
dc.subjectradiation effects
dc.subjecttoxicity
dc.subjectcarcinomas
dc.titleFlow cytometry based micronucleus assay for evaluation of genotoxic potential of 2-ACBs in hepatic cells HepG2
dc.typeArtigo de peri??dico
dc.coverageN


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