| dc.description | Sterilization by irradiation is a technique that is used by tissue banks aiming to eliminate
contamination of human allografts, being a safe method, free of residue and used as final
sterilization. After the tissue procurement, these undergo a series of processing stages and
then are packaged and preserved by freezing. Despite aseptic care of the material those may
be subjected to sterilization in the final packing by ionizing radiation, raising the security
level of sterility of the tissue. The aim of this study was to evaluate the effects of application
of ionizing radiation, produced by 60Co source in human tendons preprocessed (A-alcohol +
antibiotic; B- H2O2 + ultrasound) obtained through collaboration with tissue banks and
preserved by freezing in -80?? C, the radiation absorbed doses in processing were 12.5, 15 and
25 kGy, each one with their corresponding non-irradiated control, to examine possible
structural or morphological alterations. The irradiated samples and their controls were
analyzed by means of optical coherence tomography (OCT) and optical coherence
tomography polarization sensitive (PS-OCT), and histological tests had been stained with
hematoxylin-eosin (HE). According to the results the tissue processed with alcohol/antibiotic
in conjunction with irradiation proved to be the most effective. | |
