UVA and UVB formulation phototoxicity in a three-dimensional human skin model

dc.creatorUCO, DAYANE P.
dc.creatorLEITE-SILVA, VANIA R.
dc.creatorSILVA, HERON D.T.
dc.creatorDUQUE, MARCELO D.
dc.creatorGRICE, JEFFREY
dc.creatorMATHOR, MONICA B.
dc.creatorANDREO-FILHO, NEWTON
dc.creatorLOPES, PATRICIA S.
dc.date2018
dc.date2018-12-05T12:01:17Z
dc.date2018-12-05T12:01:17Z
dc.date.accessioned2023-09-28T14:08:53Z
dc.date.available2023-09-28T14:08:53Z
dc.identifier0887-2333
dc.identifierhttp://repositorio.ipen.br/handle/123456789/29319
dc.identifier53
dc.identifier10.1016/j.tiv.2018.07.009
dc.identifieraguardando
dc.identifier61.828
dc.identifier81.00
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8999561
dc.descriptionIn vitro three-dimensional human skin models are an innovative alternative to evaluate cytotoxicity and phototoxicity in the cosmetic industry. The aim of this study was to use a skin model to evaluate the potential toxicity of sunscreen formulations with or without exposure to UV radiation. In addition, the toxicity of these formulations was evaluated after exposure to photodegradation. The results showed toxicity with all formulations/conditions tested, including the control formulation, compared to PBS. Cell viability of photodegraded formulations - prior to the phototoxicity radiation process - was higher, indicating that some formulation components were degraded into products with reduced toxicity. The results also indicated that avobenzone was more unstable/toxic than octyl p-methoxycinnamate under the same test conditions. The sunscreens and their formulations were shown to be toxic to skin model cells to some extent, even when not exposed to UV irradiation; however the biological role of this toxicity is unclear. This result shows the importance of testing sunscreen formulations in real in-use conditions. Finally, since we used an in vitro assay based on a human cell model, this non-invasive technique represents a suitable alternative to animal models for phototoxicity tests in general and could have application in screening new sunscreen products.
dc.descriptionFunda????o de Amparo ?? Pesquisa do Estado de S??o Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico (CNPq)
dc.descriptionFAPESP: 12/07565-2
dc.descriptionCNPq: 447474/ 2014-6
dc.format37-44
dc.relationToxicology in Vitro
dc.rightsopenAccess
dc.subjectultraviolet radiation
dc.subjecttoxicity
dc.subjectspectrophotometry
dc.subjectthree-dimensional calculations
dc.subjectskin
dc.subjectepidermis
dc.subjectanimal cells
dc.subjectphotoreactivation
dc.subjectradiolysis
dc.subjectepitheliomas
dc.subjectsolar radiation
dc.titleUVA and UVB formulation phototoxicity in a three-dimensional human skin model
dc.typeArtigo de peri??dico
dc.coverageI


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