Gamma irradiation of Toxoplasma gondii protein extract improve immune response and protection in mice models

dc.creatorCOSTA, ANDREA da
dc.creatorZORGI, NAHIARA E.
dc.creatorNASCIMENTO, NANCI do
dc.creatorGALISTEO JUNIOR, ANDRES J.
dc.creatorANDRADE JUNIOR, HEITOR F. de
dc.date2018
dc.date2018-11-29T17:16:19Z
dc.date2018-11-29T17:16:19Z
dc.date.accessioned2023-09-28T14:08:47Z
dc.date.available2023-09-28T14:08:47Z
dc.identifier0753-3322
dc.identifierhttp://repositorio.ipen.br/handle/123456789/29295
dc.identifier106
dc.identifier10.1016/j.biopha.2018.06.155
dc.identifieraguardando
dc.identifier71.755
dc.identifier56.00
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8999537
dc.descriptionGamma radiation induces protein changes that enhance immunogenicity for venoms, used in antivenin production. Coccidian parasites exposed to gamma radiation elicit immune response with protection in mice and man, but without studies on the effect of gamma radiation in soluble acellular extracts or isolated proteins. Toxoplasmosis is a highly prevalent coccidian disease with only one vaccine for veterinary use but with remaining tissue cysts. Total parasite extracts or recombinant proteins used as immunogen induce usually low protection. Here, we study gamma radiation effect on T. gondii extracts proteins (STAG) and its induced immunity in experimental mice models. By SDS-PAGE, protein degradation is seen at high radiation doses, but at ideal dose (1500 Gy), there are preservation of the antigenicity and immunogenicity, detected by specific antibody recognition or production after mice immunization. Immunization with STAG irradiated at 1500 Gy induced significant protection in mice immunized and challenged with distinct T. gondii strains. In their blood, higher levels of specific CD19+, CD3+CD4+ and CD3+CD8+ activated cells were found when compared to mice immunized with STAG. Irradiated T. gondii tachyzoites extracts induce immune response and protection in mice in addition, could be a feasible alternative for Toxoplasma vaccine.
dc.descriptionFunda????o de Amparo ?? Pesquisa do Estado de S??o Paulo (FAPESP)
dc.descriptionFAPESP: 14/17029-4
dc.format599-604
dc.relationBiomedicine & Pharmacotherapy
dc.rightsopenAccess
dc.subjectgamma radiation
dc.subjectparasites
dc.subjectimmunity
dc.subjectvaccines
dc.subjectproteins
dc.subjectglycoproteins
dc.subjectimmune reactions
dc.subjectmice
dc.titleGamma irradiation of Toxoplasma gondii protein extract improve immune response and protection in mice models
dc.typeArtigo de peri??dico
dc.coverageI


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