Characterization of the europium tetracycline complex as a biomarker for atherosclerosis

dc.contributorPOPP, JURGEN
dc.contributorTUCHIN, VALERY
dc.contributorMATTHEWS, DENNIS L.
dc.contributorPAVONE, FRANCESCO S.
dc.creatorCOURROL, LILIA C.
dc.creatorSILVA, MONICA N. da
dc.creatorSICCHIERI, LETICIA B.
dc.creatorBIOPHOTONICS: PHOTONIC SOLUTIONS FOR BETTER HEALTH CARE, 5th
dc.date2017-05-29T11:16:52Z
dc.date2017-05-29T11:16:52Z
dc.dateApril 03, 2016
dc.date.accessioned2023-09-28T13:34:01Z
dc.date.available2023-09-28T13:34:01Z
dc.identifierhttp://repositorio.ipen.br/handle/123456789/27525
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8997792
dc.descriptionAtherosclerosis is a narrowing of the arteries caused by an increase of atheromatous plaque: material formed by macrophage cells containing cholesterol and fatty acids, calcium and a variable amount of fibrous connective tissue. The elation between vulnerable plaques and cardiovascular events can be determined using plaque biomarkers. In this work, atherosclerotic plaques stained with different molar ratios of europium, in a potential plaque biomarker, europium tetracycline complex, were studied by fluorescence microscopy. The tetracycline antibiotic used was chlortetracycline. The growth of atherosclerotic plaque was followed during 60 days in New Zealand rabbits divided in two groups: an experimental group (EG), with nine animals and a control group (CG) with three animals. The animals in the EG received a diet with 1% of cholesterol and the animals of GC received a normal diet. The aortic arch of the animals with 60 days were cut in the vertical plane in 6 ??m thick slices, which were mounted on glass slides and stained with hematoxylin an eosin and europium chlortetracycline complex (EuCTc). The fluorescence images were obtained exciting the EuCTc absorption band with a filter cube D (BP 355 ??? 425) and the emission was collected with a LP 470 suppression filter. Light intensity, detector gain and acquisition time were fixed for comparisons. The 20?? magnified images were collected with 12 bit (or 4096 gray tones) resolution. The mean value of gray scale for each molar ratio of EuCTc was different, indicating that the complex interacts with the components of atherosclerotic plaque and the best molar ratio was 1.5 EuCTc. These results indicate the potential use of the EuCTc biomarker for atherosclerotic plaque characterization.
dc.format98872D-1 - 98872D-7
dc.publisherBellingham, Washington USA: Society of Photo-optical Instrumentation Engineers
dc.relationSPIE Proceedings Series, 9887
dc.rightsopenAccess
dc.subjectarteriosclerosis
dc.subjectvascular diseases
dc.subjecteuropium
dc.subjecttetracyclines
dc.subjectbiological markers
dc.titleCharacterization of the europium tetracycline complex as a biomarker for atherosclerosis
dc.typeTexto completo de evento
dc.coverageI


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