Physical-chemical and biological characterization of different preparations of equine chorionic gonadotropin

dc.creatorALVAREZ, RAFAEL H.
dc.creatorNATAL, FABIO L.N.
dc.creatorRIBELA, MARIA T.C.P.
dc.creatorALMEIDA, BEATRIZ E. de
dc.creatorOLIVEIRA, JOAO E. de
dc.creatorBARTOLINI, PAOLO
dc.date2016
dc.date2017-03-13T18:37:11Z
dc.date2017-03-13T18:37:11Z
dc.date.accessioned2023-09-28T13:32:43Z
dc.date.available2023-09-28T13:32:43Z
dc.identifier1229-845X
dc.identifierhttp://repositorio.ipen.br/handle/123456789/27152
dc.identifier4
dc.identifier17
dc.identifier10.4142/jvs.2016.17.4.459
dc.identifier64.338
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8997434
dc.descriptionOvarian stimulation with commercial preparations of equine chorionic gonadotropin (eCG) produces extremely variable responses in domestic animals, ranging from excessive stimulation to practically no stimulation, when applied on the basis of their declared unitage. This study was conducted to analyze four commercial preparations from different manufacturers via reversed-phase HPLC (RP-HPLC) in comparison with a reference preparation and an official International Standard from the World Health Organization. The peaks obtained by this qualitative and quantitative physical???chemical analysis were compared using an in vivo bioassay based on the ovarian weight gain of prepubertal female rats. The RP-HPLC data showed one or two peaks close to a main peak (tR = 27.9 min), which were related to the in vivo bioactivity. Commercial preparations that have this altered peak showed very little or no in vivo activity, as demonstrated by rat ovarian weight and in peripubertal gilts induced to ovulate. Overall, these findings indicate that RP-HPLC can be a rapid and reliable tool to reveal changes in the physicochemical profile of commercial eCG that is apparently related to decreased biological activity of this hormone.
dc.descriptionFunda????o de Amparo ?? Pesquisa do Estado de S??o Paulo (FAPESP)
dc.descriptionFAPESP: 11/13096-0
dc.format459-465
dc.relationJournal of Veterinary Science
dc.rightsopenAccess
dc.subjectgonadotropins
dc.subjecthigh pressure
dc.subjectliquid column chromatography
dc.subjectbioassay
dc.subjectovaries
dc.subjectanimal cells
dc.subjecthorses
dc.titlePhysical-chemical and biological characterization of different preparations of equine chorionic gonadotropin
dc.typeArtigo de peri??dico
dc.coverageI


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