Impaired malate and fumarate accumulation due the mutation of tonoplast dicarboxylate transporter

dc.creatorMedeiros, David B.
dc.creatorBarros, Kallyne
dc.creatorBarros, Jessica AS
dc.creatorOmena-Garcia, Rebeca P
dc.creatorDetmann, Kelly C
dc.creatorSilva, Willian Batista
dc.creatorDaMatta, Fabio
dc.creatorNunes-Nesi, Adriano
dc.creatorAraújo, Wagner L.
dc.creatorArrivault, Stéphanie
dc.creatorSanglard, Lilian Vincis Pereira
dc.creatorDaloso, Danilo M.
dc.creatorFernie, Alisdair R.
dc.date2017-10-26T13:42:13Z
dc.date2017-10-26T13:42:13Z
dc.date2017-09-20
dc.date.accessioned2023-09-27T22:23:52Z
dc.date.available2023-09-27T22:23:52Z
dc.identifier15322548
dc.identifierhttps://doi.org/10.1104/pp.17.00971
dc.identifierhttp://www.locus.ufv.br/handle/123456789/12448
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8974576
dc.descriptionMalate is a central metabolite involved in a multiplicity of plant metabolic pathways, being associated with mitochondrial metabolism and playing significant roles n stomatal movements. Vacuolar malate transport has been characterized at the molecular level and is performed by at least one carrier protein and two channels in Arabidopsis vacuoles. The absence of the Arabidopsis thaliana tonoplast Dicarboxylate Transporter (tDT) in tdt knockout mutant was previously associated with an impaired accumulation of malate and fumarate in leaves. Here, we investigated the consequences of this lower accumulation on stomatal behaviour and photosynthetic capacity as well as ts putative metabolic impacts. Neither the stomatal conductance (g s ) nor the kinetic responses to dark, light or high CO 2 were highly affected in tdt plants. In addition, we did not observe any impact on stomatal aperture following incubation with either bscisic acid (ABA), malate or citrate. Further, an effect on photosynthetic capacity was not observed in the mutant lines. However, leaf mitochondrial metabolism was affected in the tdt plants. Levels of the intermediates of the tricarboxylic acid cycle were altered and increases in both light and dark respiration were observed. We conclude that manipulation of the tonoplastic organic acid transporter impacted mitochondrial metabolism, while the overall stomatal and photosynthetic capacity were unaffected.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherPlant Physiology
dc.relationVol. 175, Issue 2, Oct. 2017
dc.rightsOpen Acess
dc.subjectOrganic acids
dc.subjectGrowth
dc.subjectPrimary metabolism
dc.subjectRespiration
dc.subjectStomata
dc.titleImpaired malate and fumarate accumulation due the mutation of tonoplast dicarboxylate transporter
dc.typeArtigo


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