Otro
Histologic Characterization of Engineered Tissues in the Canal Space of Closed-apex Teeth with Apical Periodontitis
Registro en:
Journal of Endodontics.
0099-2399
10.1016/j.joen.2013.08.023
WOS:000327916700014
2-s2.0-84884538597
Autor
Gomes-Filho, João Eduardo
Tobias Duarte, Paulo Carvalho
Ervolino, Edilson
Bomfim, Suely Regina Mogami
Xavier Abimussi, Caio José
Mota da Silva Santos, Ludmilla
Lodi, Carolina Simonetti
Oliveira, Sandra Helena Penha de
Dezan Júnior, Eloi
Cintra, Luciano Tavares Angelo
Resumen
Introduction: The aim of this study was to investigate the capacity of endodontic regenerative procedures combining an induced blood clot, platelet-rich plasma (PRP), and bone marrow aspirate (BMA) to regenerate dental pulp in canine closed-apex necrotic teeth. Methods: Apical periodontitis was induced in 20 upper and lower premolars of 2 dogs. After biomechanical preparation, enlargement to a #60 file, and disinfection with a triantibiotic paste for 28 days, the roots were randomly assigned to 4 treatment groups: blood clot (BC), BC + PRP gel, BC + BMA gel, and BC + BMA/PRP gel. Negative controls were also included. After a 3-month follow-up period, the animals were killed. Results: Histologic analysis showed the presence of newly formed vital tissues (connective, cement-like, and bone-like tissue) in 23 of the 32 treated roots (71.87%). There was no statistically significant difference between the treatment groups. Conclusions: New vital tissues were formed and characterized as connective, cementum-like, or bone-like, but not as pulp-like tissue; PRP and/or BMA did not improve the tissue ingrowth. © 2013 American Association of Endodontists.