The glyceraldehyde-3-phosphate dehydrogenase gene of Moniliophthoraperniciosa, the causal agent of witches' broom disease of Theobroma cacao

dc.creatorLima, Juliana O.
dc.creatorPereira, Jorge F.
dc.creatorRincones, Johana
dc.creatorBarau, Joan G.
dc.creatorAraújo, Elza F.
dc.creatorPereira, Gonçalo A.G.
dc.creatorQueiroz, Marisa V.
dc.date2017-10-24T16:47:57Z
dc.date2017-10-24T16:47:57Z
dc.date2008-12-05
dc.date.accessioned2023-09-27T22:17:48Z
dc.date.available2023-09-27T22:17:48Z
dc.identifier1415-4757
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572009000200024
dc.identifierhttp://www.locus.ufv.br/handle/123456789/12359
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8973285
dc.descriptionThis report describes the cloning, sequence and expression analysis of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene of Moniliophthora perniciosa, the most important pathogen of cocoa in Brazil. Southern blot analysis revealed the presence of a single copy of the GAPDH gene in the M. perniciosa genome (MpGAPDH). The complete MpGAPDH coding sequence contained 1,461 bp with eight introns that were conserved in the GAPDH genes of other basidiomycete species. The cis-elements in the promoter region of the MpGAPDH gene were similar to those of other basidiomycetes. Likewise, the MpGAPDH gene encoded a putative 339 amino acid protein that shared significant sequence similarity with other GAPDH proteins in fungi, plants, and metazoans. Phylogenetic analyses clustered the MPGAPDH protein with other homobasidiomycete fungi of the family Tricholomataceae. Expression analysis of the MpGAPDH gene by real-time PCR showed that this gene was more expressed (~1.3X) in the saprotrophic stage of this hemibiotrophic plant pathogen than in the biotrophic stage when grown in cacao extracts.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherGenetics and Molecular Biology
dc.relationv.32, n.2, p.362-366 Apr.-Jun. 2009
dc.rightsOpen Access
dc.subjectGlyceraldehyde-3-phosphate dehydrogenase gene
dc.subjectMoniliophthora perniciosa
dc.subjectWitches' broom
dc.titleThe glyceraldehyde-3-phosphate dehydrogenase gene of Moniliophthoraperniciosa, the causal agent of witches' broom disease of Theobroma cacao
dc.typeArtigo


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