Sucrose breakdown within guard cells provides substrates for glycolysis and glutamine biosynthesis during light- induced stomatal opening

dc.creatorMedeiros, David B.
dc.creatorSouza, Leonardo Perez
dc.creatorAntunes, Werner C.
dc.creatorAraújo, Wagner L.
dc.creatorDaloso, Danilo M.
dc.creatorFernie, Alisdair R.
dc.date2019-04-11T16:44:49Z
dc.date2019-04-11T16:44:49Z
dc.date2018-05
dc.date.accessioned2023-09-27T22:17:34Z
dc.date.available2023-09-27T22:17:34Z
dc.identifier1365-313X
dc.identifierhttps://doi.org/10.1111/tpj.13889
dc.identifierhttp://www.locus.ufv.br/handle/123456789/24494
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8973236
dc.descriptionSucrose has long been thought to play an osmolytic role in stomatal opening. However, recent evidence supports the idea that the role of sucrose in this process is primarily energetic. Here we used a combination of stomatal aperture assays and kinetic [U- 13 C]-sucrose isotope labelling experiments to confirm that sucrose is degraded during light-induced stomatal opening and to define the fate of the C released from sucrose breakdown. We additionally show that addition of sucrose to the medium did not enhance light- induced stomatal opening. The isotope experiment showed a consistent 13 C enrichment in fructose and glu- cose, indicating that during light-induced stomatal opening sucrose is indeed degraded. We also observed a clear 13 C enrichment in glutamate and glutamine (Gln), suggesting a concerted activation of sucrose degra- dation, glycolysis and the tricarboxylic acid cycle. This is in contrast to the situation for Gln biosynthesis in leaves under light, which has been demonstrated to rely on previously stored C. Our results thus collectively allow us to redraw current models concerning the influence of sucrose during light-induced stomatal open- ing, in which, instead of being accumulated, sucrose is degraded providing C skeletons for Gln biosynthesis.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherThe Plant Journal
dc.relationv. 94, n. 4, p. 583-594, maio 2018
dc.rightsOpen Access
dc.subjectStomatal movements
dc.subjectSucrose
dc.subjectGuard cell metabolism
dc.subjectTCA cycle
dc.subjectGlycolysis
dc.subjectStable isotope label- ling analysis
dc.titleSucrose breakdown within guard cells provides substrates for glycolysis and glutamine biosynthesis during light- induced stomatal opening
dc.typeArtigo


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