Chromosomal diversification of diploid number, heterochromatin and rDNAs in two species of Phanaeus beetles (Scarabaeidae, Scarabaeinae)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorArcanjo, Amanda
dc.creatorCabral-de-Mello, Diogo Cavalcanti
dc.creatorMartins, Cesar
dc.creatorMoura, Rita de Cássia de
dc.creatorSouza, Maria José de
dc.date2014-05-27T11:30:40Z
dc.date2016-10-25T18:53:59Z
dc.date2014-05-27T11:30:40Z
dc.date2016-10-25T18:53:59Z
dc.date2013-09-05
dc.date.accessioned2017-04-06T02:38:43Z
dc.date.available2017-04-06T02:38:43Z
dc.identifierGenetics and Molecular Biology, v. 36, n. 3, p. 341-346, 2013.
dc.identifier1415-4757
dc.identifier1678-4685
dc.identifierhttp://hdl.handle.net/11449/76529
dc.identifierhttp://acervodigital.unesp.br/handle/11449/76529
dc.identifier10.1590/S1415-47572013005000031
dc.identifierS1415-47572013005000031
dc.identifierWOS:000323725500007
dc.identifier2-s2.0-84883275469.pdf
dc.identifier2-s2.0-84883275469
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572013005000031
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/897223
dc.descriptionThe genus Phanaeus is included in the tribe Phanaeini, one of the most diverse tribes within the subfamily Scarabaeinae in terms of chromosomal characteristics. However, so far the species of this genus were not studied with differential cytogenetic techniques, limiting any inference of the probable mechanisms responsible for this diversity. In this work, several techniques were applied with the aim of cytogenetically characterizing two Phanaeus species. The karyotype found for Phanaeus (Notiophanaeus) chalcomelas was 2n = 12, neo-XY, and that of P. (N.) splendidulus was 2n = 20, Xyp, considered primitive for the family Scarabaeidae. The chromosomes of both species showed a high amount of constitutive heterochromatin (CH), with blocks rich in base pairs GC (CMA3 +). Moreover, in P. (N.) chalcomelas the marks revealed by C-banding and fluorochrome staining were different in size, showing CH variability. Sites of 18S ribosomal DNA (rDNA) were identified in one autosomal pair of P. (N.) chalcomelas and in five autosomal pairs of P. (N.) splendidulus. On the other hand, only one autosomal pair exhibited 5S rDNA sequences in these species. The results suggest that the karyotype differentiation of the Phanaeus species studied here involved pericentric inversions and centric fusions, as well as mechanisms related to amplification and dispersion of CH and rDNA sequences. © 2013, Sociedade Brasileira de Genética.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.relationGenetics and Molecular Biology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChromosome evolution
dc.subjectFISH
dc.subjectKaryotype
dc.subjectRepetitive DNA
dc.subjectDNA 18S
dc.subjectDNA 5S
dc.subjectribosome DNA
dc.subjectanimal tissue
dc.subjectautosome
dc.subjectbase pairing
dc.subjectbeetle
dc.subjectC banding
dc.subjectchromosomal diversification
dc.subjectchromosome NOR
dc.subjectchromosomes and chromosomal phenomena
dc.subjectdiploidy
dc.subjectDNA sequence
dc.subjectfluorescence in situ hybridization
dc.subjectfluorochrome staining
dc.subjectgene mapping
dc.subjectheterochromatin
dc.subjectkaryotype
dc.subjectmale
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpericentric chromosome inversion
dc.subjectPhanaeus
dc.subjectPhanaeus (Notiophanaeus) chalcomelas
dc.subjectPhanaeus (Notiophanaeus) splendidulus
dc.titleChromosomal diversification of diploid number, heterochromatin and rDNAs in two species of Phanaeus beetles (Scarabaeidae, Scarabaeinae)
dc.typeOtro


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