Biocompatibility of Ferrara intracorneal ring segment with and without chondroitin sulfate coating: Clinical and histopathological evaluation in rabbits

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorAndreghetti, Eduardo
dc.creatorHashimoto, Miriam
dc.creatorDomingues, Maria Aparecida Custódio
dc.creatorAntunes, Vitor Andrigheti Coronado
dc.creatorSegundo, Paulo de Souza
dc.creatorSilva, Maria Rosa Bet de Moraes
dc.date2014-05-27T11:30:35Z
dc.date2016-10-25T18:53:41Z
dc.date2014-05-27T11:30:35Z
dc.date2016-10-25T18:53:41Z
dc.date2013-09-01
dc.date.accessioned2017-04-06T02:37:36Z
dc.date.available2017-04-06T02:37:36Z
dc.identifierActa Cirurgica Brasileira, v. 28, n. 9, p. 632-640, 2013.
dc.identifier0102-8650
dc.identifier1678-2674
dc.identifierhttp://hdl.handle.net/11449/76466
dc.identifierhttp://acervodigital.unesp.br/handle/11449/76466
dc.identifier10.1590/S0102-86502013000900002
dc.identifierS0102-86502013000900002
dc.identifierWOS:000324216400002
dc.identifier2-s2.0-84883645775.pdf
dc.identifier2-s2.0-84883645775
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502013000900002
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/897163
dc.descriptionPURPOSE: To investigate and compare the biocompatibility of two types of Ferrara intracorneal ring segment: with and without chondroitin sulfate coating by clinical and histopathological evaluation. METHODS: A randomized experimental study was carried out on thirty right-eye corneas from 30 Norfolk albino rabbits allocated into two experimental groups: Group G1 - implanted with Ferrara intracorneal ring segment without coating (FICRS) and Group G2 - implanted with Ferrara intracorneal ring segment with chondroitin sulfate coating (FICRS-CS). Left eyes formed the control group. Clinical parameters analyzed were: presence of edema, vascularization, infection and ring extrusion one, 30, and 60 days after surgery. Histopathological parameters analyzed were: number of corneal epithelial layers over and adjacent to the ring, presence of spongiosis, hydropic degeneration, basement membrane thinning, inflammatory cells, neovascularization and pseudocapsule formation. RESULTS: At clinical examination 60 days after implant, edema, vascularization and extrusion were observed respectively in 20%, 26.7%, 6.7% of FICRS corneas and in 6.7%, 6.7%, and 0% of FICRS-CS corneas. Histopathological evaluation showed epithelial-layer reduction from 5 (5;6) to 3 (3;3) with FICRS and from 5 (5;5) to 4 (3;5) with FICRS-CS in the region over the ring. Epithelial spongiosis, hydropic degeneration, and basement membrane thinning were present in 69.2%, 53.8%, and 69.2% of FICRS and in 73.3%, 73.3%, and 46.7% with FICRS-CS, respectively. Vascularization was present in 38.5% of FICRS and 13.3% with FICRS-CS, inflammatory cells in 75% of FICRS and 33.3% with FICRS-CS, and pseudocapsule in 66.7% of FICRS and 93.3% with FICRS-CS. Giant cells occurred only in the FICRS-CS group (20%). CONCLUSION: Ferrara intracorneal rings coated with chondroitin sulfate (FICRS-CS) caused lower frequency of clinical and histopathological alterations than Ferrara intracorneal rings without the coating (FICRS), demonstrating higher biocompatibility of the FICRS-CS.
dc.languageeng
dc.relationActa Cirúrgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChondroitin sulfates
dc.subjectCornea
dc.subjectCorneal neovascularization
dc.subjectProsthesis implantation
dc.subjectRabbits
dc.subjectchondroitin sulfate
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectbiocompatibility
dc.subjectcontrolled study
dc.subjectcornea neovascularization
dc.subjectcorneal pachymetry
dc.subjectedema
dc.subjectepithelium cell
dc.subjectfemale
dc.subjectgiant cell
dc.subjecthistopathology
dc.subjectinflammatory cell
dc.subjectintrastromal corneal ring segment
dc.subjectnonhuman
dc.subjectpostoperative complication
dc.subjectrabbit
dc.titleBiocompatibility of Ferrara intracorneal ring segment with and without chondroitin sulfate coating: Clinical and histopathological evaluation in rabbits
dc.typeOtro


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