Analysis of inteins in the Candida parapsilosis complex for simple and accurate species identification

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorPrandini, Tâmara Heloísa Rocha
dc.creatorTheodoro, Raquel Cordeiro
dc.creatorBruder-Nascimento, Ariane C. M. O.
dc.creatorScheel, Christina M.
dc.creatorBagagli, Eduardo
dc.date2014-05-27T11:30:34Z
dc.date2016-10-25T18:53:36Z
dc.date2014-05-27T11:30:34Z
dc.date2016-10-25T18:53:36Z
dc.date2013-09-01
dc.date.accessioned2017-04-06T02:37:19Z
dc.date.available2017-04-06T02:37:19Z
dc.identifierJournal of Clinical Microbiology, v. 51, n. 9, p. 2830-2836, 2013.
dc.identifier0095-1137
dc.identifier1098-660X
dc.identifierhttp://hdl.handle.net/11449/76454
dc.identifierhttp://acervodigital.unesp.br/handle/11449/76454
dc.identifier10.1128/JCM.00981-13
dc.identifierWOS:000323214200005
dc.identifier2-s2.0-84882736473.pdf
dc.identifier2-s2.0-84882736473
dc.identifierhttp://dx.doi.org/10.1128/JCM.00981-13
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/897153
dc.descriptionInteins are coding sequences that are transcribed and translated with flanking sequences and then are excised by an autocatalytic process. There are two types of inteins in fungi, mini-inteins and full-length inteins, both of which present a splicing domain containing well-conserved amino acid sequences. Full-length inteins also present a homing endonuclease domain that makes the intein a mobile genetic element. These parasitic genetic elements are located in highly conserved genes and may allow for the differentiation of closely related species of the Candida parapsilosis (psilosis) complex. The correct identification of the three psilosis complex species C. parapsilosis, Candida metapsilosis, and Candida orthopsilosis is very important in the clinical setting for improving antifungal therapy and patient care. In this work, we analyzed inteins that are present in the vacuolar ATPase gene VMA and in the threonyl-tRNA synthetase gene ThrRS in 85 strains of the Candida psilosis complex (46 C. parapsilosis, 17 C. metapsilosis, and 22 C. orthopsilosis). Here, we describe an accessible and accurate technique based on a single PCR that is able to differentiate the psilosis complex based on the VMA intein. Although the ThrRS intein does not distinguish the three species of the psilosis complex by PCR product size, it can differentiate them by sequencing and phylogenetic analysis. Furthermore, this intein is unusually present as both mini- and full-length forms in C. orthopsilosis. Additional population studies should be performed to address whether this represents a common intraspecific variability or the presence of subspecies within C. orthopsilosis. Copyright © 2013, American Society for Microbiology. All Rights Reserved.
dc.languageeng
dc.relationJournal of Clinical Microbiology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectintein
dc.subjectthreonine transfer RNA ligase
dc.subjectaccuracy
dc.subjectCandida metapsilosis
dc.subjectCandida orthopsilosis
dc.subjectCandida parapsilosis
dc.subjectcladistics
dc.subjectcontrolled study
dc.subjectfungal gene
dc.subjectfungal strain
dc.subjectfungus identification
dc.subjectfungus isolation
dc.subjectgene function
dc.subjectgene identification
dc.subjectmolecular phylogeny
dc.subjectnonhuman
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectprotein determination
dc.subjectprotein function
dc.subjectsequence analysis
dc.subjectthreonyl tRNA synthetase gene
dc.subjectvacuolar ATPase gene
dc.titleAnalysis of inteins in the Candida parapsilosis complex for simple and accurate species identification
dc.typeOtro


Este ítem pertenece a la siguiente institución