Transdentinal cytotoxicity of experimental adhesive systems of different hydrophilicity applied to ethanol-saturated dentin

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBianchi, Luciana
dc.creatorRibeiro, Ana Paula Dias
dc.creatorDe Oliveira Carrilho, Marcela Rocha
dc.creatorPashley, David H.
dc.creatorDe Souza Costa, Carlos Alberto
dc.creatorHebling, Josimeri
dc.date2014-05-27T11:30:32Z
dc.date2016-10-25T18:53:08Z
dc.date2014-05-27T11:30:32Z
dc.date2016-10-25T18:53:08Z
dc.date2013-09-01
dc.date.accessioned2017-04-06T02:36:46Z
dc.date.available2017-04-06T02:36:46Z
dc.identifierDental Materials, v. 29, n. 9, p. 980-990, 2013.
dc.identifier0109-5641
dc.identifierhttp://hdl.handle.net/11449/76399
dc.identifierhttp://acervodigital.unesp.br/handle/11449/76399
dc.identifier10.1016/j.dental.2013.07.006
dc.identifierWOS:000323561600015
dc.identifier2-s2.0-84882456639
dc.identifierhttp://dx.doi.org/10.1016/j.dental.2013.07.006
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/897099
dc.descriptionThe aim of this study was to evaluate the transdentinal cytotoxicity of experimental adhesive systems (EASs) with different hydrophilicity and dentin saturation solutions on odontoblast-like cells. One hundred 0.4-mm-thick dentin discs were mounted in in vitro pulp chambers and assigned to 10 groups. MDPC-23 cells were seeded onto the pulpal side of the discs, incubated for 48 h. The EASs with increasing hydrophilicity (R1, R2, R3 and R4) were applied to the occlusal side after etching and saturation of etched dentin with water or ethanol. R0 (no adhesive) served as controls. R1 is a non-solvated hydrophobic blend, R2 is similar to a simplified etch-and-rinse adhesive system and R3 and R4 are similar to self-etching adhesives. After 24 h, cell metabolism was evaluated by MTT assay (n = 8 discs) and cell morphology was examined by SEM (n = 2 discs). Type of cell death was identified by flow cytometry and the degree of monomer conversion (%DC) was determined by infrared spectroscopy (FTIR) after 10 s or 20 s of photoactivation. Data were analyzed by the Kruskal-Wallis and Mann-Whitney tests (α = 0.05). Dentin saturation with ethanol resulted in higher necrotic cell death ratios for R2, R3 and R4 compared with water saturation, although R2 and R3 induced higher SDH production. Photoactivation for 20 s significantly improved the %DC of all EASs compared with 10 s. A significant positive correlation was observed between the degree of hydrophilicity and %DC. In conclusion, except for R1, dentin saturation with ethanol increased the cytotoxicity of EASs, as expressed by the induction of necrotic cell death. © 2013 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
dc.languageeng
dc.relationDental Materials
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAdhesive systems
dc.subjectCytotoxicity
dc.subjectDentin
dc.subjectEthanol
dc.subjectOdontoblast-like cells
dc.subjectMonomer conversions
dc.subjectNecrotic cell deaths
dc.subjectPhoto activations
dc.subjectPositive correlations
dc.subjectSelf-etching adhesives
dc.subjectWater saturations
dc.subjectCell death
dc.subjectEtching
dc.subjectHydrophilicity
dc.subjectInfrared spectroscopy
dc.titleTransdentinal cytotoxicity of experimental adhesive systems of different hydrophilicity applied to ethanol-saturated dentin
dc.typeOtro


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